Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Structure and Composition of Native Membrane Derived Polymer-Supported Lipid Bilayers
Chalmers University of Technology, Sweden.
RISE - Research Institutes of Sweden, Bioscience and Materials, Surface, Process and Formulation. Chalmers University of Technology, Sweden.
Chalmers University of Technology, Sweden.
Institute Laue-Langevin, France ; Imperial College London, UK.
Show others and affiliations
2018 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 21, p. 13065-13072Article in journal (Refereed) Published
Abstract [en]

Over the last two decades, supported lipid bilayers (SLBs) have been extensively used as model systems to study cell membrane structure and function. While SLBs have been traditionally produced from simple lipid mixtures, there has been a recent surge in compositional complexity to better mimic cellular membranes and thereby bridge the gap between classic biophysical approaches and cell experiments. To this end, native cellular membrane derived SLBs (nSLBs) have emerged as a new category of SLBs. As a new type of biomimetic material, an analytical workflow must be designed to characterize its molecular composition and structure. Herein, we demonstrate how a combination of fluorescence microscopy, neutron reflectometry, and secondary ion mass spectrometry offers new insights on structure, composition, and quality of nSLB systems formed using so-called hybrid vesicles, which are a mixture of native membrane material and synthetic lipids. With this approach, we demonstrate that the nSLB formed a continuous structure with complete mixing of the synthetic and native membrane components and a molecular stoichiometry that essentially mirrors that of the hybrid vesicles. Furthermore, structural investigation of the nSLB revealed that PEGylated lipids do not significantly thicken the hydration layer between the bilayer and substrate when on silicon substrates; however, nSLBs do have more topology than their simpler, purely synthetic counterparts. Beyond new insights regarding the structure and composition of nSLB systems, this work also serves to guide future researchers in producing and characterizing nSLBs from their cellular membrane of choice.

Place, publisher, year, edition, pages
2018. Vol. 90, no 21, p. 13065-13072
Keywords [en]
Biomimetics, Cell membranes, Cytology, Fluorescence microscopy, Lipid bilayers, Lipids, Mixtures, Secondary ion mass spectrometry, Substrates, Cellular membranes, Compositional complexity, Continuous structures, Molecular compositions, Neutron reflectometry, Silicon substrates, Structural investigation, Supported lipid bilayers, Structure (composition)
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-36374DOI: 10.1021/acs.analchem.8b04110Scopus ID: 2-s2.0-85056285757OAI: oai:DiVA.org:ri-36374DiVA, id: diva2:1266232
Available from: 2018-11-27 Created: 2018-11-27 Last updated: 2019-06-20Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records BETA

Sjövall, Peter

Search in DiVA

By author/editor
Sjövall, Peter
By organisation
Surface, Process and Formulation
In the same journal
Analytical Chemistry
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 6 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
v. 2.35.7