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Silica-based diffusion probes for use in FRAP and NMR-diffusometry
Chalmers University of Technology, Sweden.
Chalmers University of Technology, Sweden.
Chalmers University of Technology, Sweden.
Chalmers University of Technology, Sweden.
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2019 (English)In: Journal of Dispersion Science and Technology, ISSN 0193-2691, E-ISSN 1532-2351, Vol. 40, no 4, p. 555-562Article in journal (Refereed) Published
Abstract [en]

Development of multi-purpose probes for mass transport measurements is of importance to gain knowledge in diffusional behaviour in heterogeneous structures such as food, hygiene or pharamceuticals. By combining different techniques, such as Fluorescence Recovery After Photobleaching (FRAP) and Nuclear Magnetic Resonance Diffusometry (NMR-d), information of both local and global diffusion can be collected and used to gain insights on for example material heterogeneities and probe-material interactions. To obtain a FRAP-responsive probe, fluorescent silica particles were produced using fluorescent preconjugates added in a modified Stöber process. A NMR-d responsive moiety was introduced by derivatizing the fluorescent silica particles with polyethylene glycol. The particle size distributions were determined by dynamic light scattering and transmission electron microscopy and these measurements were compared to value extrapolated from diffusion measurements using FRAP and NMR-d. The good agreement between the FRAP and NMR-d measurements demonstrates the potential of multi-purpose probes for future applications concerning mass transport at local and global scale simultaneously. © 2018, © 2018 The Author(s).

Place, publisher, year, edition, pages
2019. Vol. 40, no 4, p. 555-562
Keywords [en]
Diffusion probes, fluorescent silica nanoparticles, FRAP, NMR-diffusometry, pegylated silica nanoparticles, Diffusion, Fluorescence, High resolution transmission electron microscopy, Light scattering, Light transmission, Particle size, Photobleaching, Probes, Silica nanoparticles, Transmission electron microscopy, Diffusion measurements, Fluorescence recovery after photobleaching, Heterogeneous structures, NMR diffusometry, Pegylated, Transport measurements, Nuclear magnetic resonance
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Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-35935DOI: 10.1080/01932691.2018.1472015Scopus ID: 2-s2.0-85054577994OAI: oai:DiVA.org:ri-35935DiVA, id: diva2:1261068
Available from: 2018-11-06 Created: 2018-11-06 Last updated: 2019-07-01Bibliographically approved

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Loren, Niklas

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