The aim of the work was to evaluate novel biomixtures for their use on biopurification systems (BPS) in Argentina also called biobeds. Glyphosate and aminomethylphosphonic acid (AMPA) degradation was evaluated on biomixtures containing local materials: alfalfa straw (As), wheat stubble (Ws), river waste (Rw) and soil. Glyphosate, AMPA concentrations and biological activity were followed with time. Soil was used as control. Glyphosate initial concentration was 1000 mg kg-1. Glyphosate disappeared almost completely after 63 days in all tested biomixtures. For Ws, WsRw and AsRw glyphosate degradation was around 99% and for As 85%. The biomixture Ws showed the highest glyphosate degradation rate. In all cases AMPA was formed and degraded to concentrations between 60 and 100 mg kg-1. In the control with only soil, glyphosate was degraded 53% and AMPA concentration at the end of the test was 438 mg kg-1. We conclude that alfalfa straw, wheat stubble and river waste are local materials that can be used in the preparation of biomixtures since they showed higher glyphosate degradation capacity and less AMPA accumulation compared to the soil alone. Also, the presence of river waste did enhance the water retention capacity.