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Structural Analysis of Polysaccharide Networks by Transmission Electron Microscopy: Comparison with Small-Angle X-ray Scattering.
Institute of Fundamental Sciences,New Zealand ; The MacDiarmid Institute for Advanced Materials and Nanotechnology, New Zealand ; Riddet Insitute, New Zealand.
Institute of Fundamental Sciences,New Zealand ; The MacDiarmid Institute for Advanced Materials and Nanotechnology, New Zealand.
The University of Melbourne, Australia.
RISE - Research Institutes of Sweden, Bioscience and Materials, Agrifood and Bioscience.
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2018 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 19, no 3, p. 989-995Article in journal (Refereed) Published
Abstract [en]

Polysaccharide gels assembled from the anionic biopolymers pectin and carrageenan have been studied using transmission electron microscopy (TEM). Gels were formed in several different ways: for pectin, hydrogen bonding was used to form junction zones between strands, whereas for carrageenan systems, several different ion types were used to form ionotropic networks. Using this approach, several distinct network architectures were realized. In addition to preparing gelled samples for electron microscopy, a set of samples was taken without performing the additional treatment necessitated by the TEM measurements, and these were studied directly by small-angle X-ray scattering (SAXS). Taking careful consideration of the relative merits of different image sizes and available processing techniques, the real-space images acquired by TEM were used via radial integration of the Fourier transform to produce simulated scattering patterns. These intensity-versus-wavevector plots were compared with the results of SAXS experiments carried out on the unadulterated gels using synchrotron radiation. Although information regarding chain thicknesses and flexibilities was found to be modified by labeling and changes in the dielectric constant and mechanical properties of the surroundings in the TEM, the studies carried out here show that careful protocols can produce data sets where information acquired above ∼20 nm is broadly consistent with that obtained by SAXS studies carried out on unadulterated samples. The fact that at larger length scale the structure of these water-rich networks seems largely preserved in the TEM samples suggests that three-dimensional (3D) TEM tomography experiments carried out with careful sample preparation will be valuable tools for measuring network architecture and connectivity; information that is lost in SAXS owing to the intrinsic averaging nature of the technique.

Place, publisher, year, edition, pages
2018. Vol. 19, no 3, p. 989-995
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Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-33339DOI: 10.1021/acs.biomac.7b01773PubMedID: 29381344OAI: oai:DiVA.org:ri-33339DiVA, id: diva2:1186581
Available from: 2018-02-28 Created: 2018-02-28 Last updated: 2018-04-04Bibliographically approved

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