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Cytocompatibility of Wood-Derived Cellulose Nanofibril Hydrogels with Different Surface Chemistry
University of Bergen, Norway.
University of Bergen, Norway.
RISE - Research Institutes of Sweden, Bioeconomy, PFI.
RISE - Research Institutes of Sweden, Bioeconomy, PFI. Norwegian University of Science and Technology (NTNU), Norway.
2017 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 18, no 4, p. 1238-1248Article in journal (Refereed) Published
Abstract [en]

The current study aims to demonstrate the influence of the surface chemistry of wood-derived cellulose nanofibril (CNF) hydrogels on fibroblasts for tissue engineering applications. TEMPO-mediated oxidation or carboxymethylation pretreatments were employed to produce hydrogels with different surface chemistry. This study demonstrates the following: first, the gelation of CNF with cell culture medium and formation of stable hydrogels with improved rheological properties; second, the response of mouse fibroblasts cultured on the surface of the hydrogels or sandwiched within the materials with respect to cytotoxicity, cell attachment, proliferation, morphology, and migration. Indirect cytotoxicity tests showed no toxic effect of either hydrogel. The direct contact with the carboxymethylated hydrogel adversely influenced the morphology of the cells and limited their spreading, while typical morphology and spreading of cells were observed with the TEMPO-oxidized hydrogel. The porous fibrous structure may be a key to cell proliferation and migration in the hydrogels.

Place, publisher, year, edition, pages
2017. Vol. 18, no 4, p. 1238-1248
Keyword [en]
Cell culture, Cell proliferation, Cells, Cellulose, Cytology, Fibroblasts, Gelation, Morphology, Nanofibers, Surface chemistry, Tissue engineering, Wood, Carboxymethylation, Cell culture mediums, Cytotoxicity test, Fibrous structures, Rheological property, TEMPO-mediated oxidation, Tissue engineering applications, Typical morphology, Hydrogels, cross linking reagent, divalent cation, lactate dehydrogenase, biomaterial, hydrogel, nanomaterial, animal cell, Article, atomic force microscopy, biocompatibility, bioprinting, cell adhesion, cell encapsulation, cell interaction, cell migration, cell migration assay, cell structure, cell viability, chemical bond, controlled study, cross linking, culture medium, cytocompatibility, cytoskeleton, cytotoxicity, extracellular matrix, fibroblast, hydrogen bond, methylation, mouse, MTT assay, nonhuman, oxidation, priority journal, scanning electron microscopy, surface property, tissue culture, wood derived cellulose nanofibril hydrogel, animal, cell motion, cell survival, chemistry, flow kinetics, infrared spectroscopy, porosity, Surface Properties, Animals, Biocompatible Materials, Cell Movement, Cells, Cultured, Mice, Nanostructures, Rheology, Spectroscopy, Fourier Transform Infrared
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Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-33166DOI: 10.1021/acs.biomac.6b01911OAI: oai:DiVA.org:ri-33166DiVA: diva2:1176692
Note

 Funding details: AFM, Association Française contre les Myopathies; Funding details: 245963/F50; Funding details: 302077, Helse Vest;Funding details: NTNU, National Taiwan Normal University; Funding details: 228147, Norges Forskningsråd

Available from: 2018-01-23 Created: 2018-01-23 Last updated: 2018-01-23Bibliographically approved

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