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Use of multiple-locusvariable-numberof tandem repeatsanalysis (MLVA) to investigate genetic diversity of Salmonellaenterica subsp. enterica serovar Typhimurium isolates fromhuman, food, and veterinary sources
National Diagnostic Research Veterinary Institute, Sofia, Bulgaria.
National Veterinary Institute, Technical University of Denmark.
National Food Institute, Technical University of Denmark.
National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
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2017 (English)In: MicrobiologyOpen, ISSN 2045-8827, E-ISSN 2045-8827Article in journal (Refereed) Epub ahead of print
Abstract [en]

Salmonella enterica subspecies enterica serovar Typhimurium is the most common zoonotic pathogen in Bulgaria. To allow efficient outbreak investigations and surveillance in the food chain, accurate and discriminatory methods for typing are needed. This study evaluated the use of multiple-locus variable-number of tandem repeats analysis (MLVA) and compared results with antimicrobial resistance (AMR) determinations for 100 S. Typhimurium strains isolated in Bulgaria during 2008–2012 (50 veterinary/ food and 50 human isolates). Results showed that isolates were divided into 80 and 34 groups using MLVA and AMR, respectively. Simpson’s index of diversity was determined to 0.994 ± 0.003 and 0.945 ± 0.012. The most frequently encountered MLVA profiles were 3-11-9-NA-211 (n = 5); 3-12-9-NA-211 (n = 3); 3-12-11-21-311 (n = 3); 3-17-10-NA-311 (n = 3); 2-20-9-7-212 (n = 3); and 2-23-NA-NA-111 (n = 3). No clustering of isolates related to susceptibility/resistance to antimicrobials, source of isolation, or year of isolation was observed. Some MLVA types were found in both human and veterinary/food isolates, indicating a possible route of transmission. A majority (83%) of the isolates were found to be resistant against at least one antimicrobial and 44% against ≥4 antimicrobials. Further studies are needed to verify MLVA usefulness over a longer period of time and with more isolates, including outbreak strains.

Place, publisher, year, edition, pages
2017.
Keyword [en]
antimicrobial resistance, laboratory surveillance, MLVA, public health, Salmonella genetic
National Category
Microbiology
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URN: urn:nbn:se:ri:diva-31195DOI: 10.1002/mbo3.528OAI: oai:DiVA.org:ri-31195DiVA: diva2:1135709
Available from: 2017-08-24 Created: 2017-08-24 Last updated: 2017-08-25Bibliographically approved

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