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Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 86, p. 420-425Article in journal (Refereed) Published
Abstract [en]

Because of the serological cross-reactivity among the flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbreak. However, due to the limited sensitivity, the detection window of RT-PCR for Zika viremia is only about one week after symptom onset. By combining loop-mediated isothermal amplification (LAMP) and AC susceptometry, we demonstrate a rapid and homogeneous detection system for the Zika virus oligonucleotide. Streptavidin-magnetic nanoparticles (streptavidin-MNPs) are premixed with LAMP reagents including the analyte and biotinylated primers, and their hydrodynamic volumes are dramatically increased after a successful LAMP reaction. Analyzed by a portable AC susceptometer, the changes of the hydrodynamic volume are probed as Brownian relaxation frequency shifts, which can be used to quantify the Zika virus oligonucleotide. The proposed detection system can recognize 1 aM synthetic Zika virus oligonucleotide in 20% serum with a total assay time of 27 min, which can hopefully widen the detection window for Zika viremia and is therefore promising in worldwide Zika fever control.

Place, publisher, year, edition, pages
2016. Vol. 86, p. 420-425
Keywords [en]
AC susceptometer, Brownian relaxation, Loop-mediated isothermal amplification, Magnetic nanoparticles, Zika virus, Amplification, Chemical reactions, Fluid dynamics, Hydrodynamics, Isotherms, Nanomagnetics, Nanoparticles, Oligonucleotides, Proteins, Viruses, Brownian relaxations, Homogeneous detections, Hydrodynamic volumes, Loop mediated isothermal amplifications, Magnetic nano-particles, Oligonucleotide detection, Reverse transcription-polymerase chain reaction, Polymerase chain reaction, magnetic nanoparticle, AC susceptometry, agar gel electrophoresis, Article, bioassay, cross reaction, human, incubation time, infection control, limit of detection, loop mediated isothermal amplification, nucleotide sequence, process optimization, reverse transcription polymerase chain reaction, RNA extraction, scanning electron microscopy, serology, virus detection, virus load, Zika fever
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Computer and Information Sciences
Identifiers
URN: urn:nbn:se:ri:diva-27691DOI: 10.1016/j.bios.2016.06.085Scopus ID: 2-s2.0-84978245055OAI: oai:DiVA.org:ri-27691DiVA, id: diva2:1131695
Available from: 2017-10-12 Created: 2016-12-22 Last updated: 2018-01-13Bibliographically approved

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