Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cubosomes post-loaded with antimicrobial peptides: Characterization, bactericidal effect and proteolytic stability
RISE - Research Institutes of Sweden, Bioscience and Materials, Chemistry, Materials and Surfaces.
INSERM U 1066, ‘Micro et Nanomédecines biomimétiques − MINT’, Angers, France ; Université Angers, Angers, France.
INSERM U 1066, ‘Micro et Nanomédecines biomimétiques − MINT’, Angers, France ; Université Angers, Angers, France.
RISE - Research Institutes of Sweden, Bioscience and Materials, Chemistry, Materials and Surfaces.
Show others and affiliations
2017 (English)In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 526, no 1-2, 400-412 p.Article in journal (Refereed) Published
Abstract [en]

Novel antibiotics, such as antimicrobial peptides (AMPs), have recently attended more and more attraction. In this work, dispersed cubic liquid crystalline gel (cubosomes) was used as drug delivery vehicles for three AMPs (AP114, DPK-060 and LL-37). Association of peptides onto cubosomes was studied at two cubosome/peptide ratios using high performance liquid chromatography, ζ-potential and circular dichroism measurements. AMPs impact on the cubosome structure was investigated using small angle x-ray scattering and cryogenic transmission electron microscopy. The antimicrobial effect of the AMP loaded cubosomes was studied in vitro by minimum inhibitory concentration and time-kill assays. Proteolytic protection was investigated by incubating the formulations with two elastases and the antimicrobial effect after proteolysis was studied using radial diffusion assay. Different association efficacy onto the cubosomes was observed among the AMPs, with LL-37 showing greatest association (>60%). AP114 loaded cubosomes displayed a preserved antimicrobial effect, whereas for LL-37 the broad spectrum bacterial killing was reduced to only comprise Gram-negative bacteria. Interestingly, DPK-060 loaded cubosomes showed a slight enhanced effect against S. aureus and E. coli strains. Moreover, the cubosomes were found to protect LL-37 from proteolytic degradation, resulting in a significantly better bactericidal effect after being subjected to elastase, compared to unformulated peptide.

Place, publisher, year, edition, pages
Elsevier B.V. , 2017. Vol. 526, no 1-2, 400-412 p.
Keyword [en]
Cubosome; Liquid crystalline nanoparticle; Glycerol monooleate; Antimicrobial peptide; Amp; Proteolysis
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-30031DOI: 10.1016/j.ijpharm.2017.04.082Scopus ID: 2-s2.0-85019229976OAI: oai:DiVA.org:ri-30031DiVA: diva2:1119685
Available from: 2017-07-04 Created: 2017-07-04 Last updated: 2017-07-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus
By organisation
Chemistry, Materials and Surfaces
In the same journal
International Journal of Pharmaceutics
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
v. 2.26.0