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Micro-electromechanical affinity sensor for the monitoring of glucose in bioprocess media
Technical University Berlin, Germany.
RISE - Research Institutes of Sweden, ICT, Acreo. Technical University Berlin, Germany.
Technical University Berlin, Germany.
Technical University Berlin, Germany.
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2017 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, Vol. 18, no 6Article in journal (Refereed) Published
Abstract [en]

An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in the measurement cavity of the sensor probe. The study aimed to elucidate the interactions between the assay and a typical phosphate buffered bacterial cultivation medium. It turned out that contact with the medium resulted in a significant long-lasting drift of the assay’s viscosity at zero glucose concentration. Adding glucose to the medium lowers the drift by a factor of eight. The cglc values measured off-line with the glucose sensor for monitoring of a bacterial cultivation were similar to the measurements with an enzymatic assay with a difference of less than ±0.15 g·L−1. We propose that lectin agglomeration, the electro-viscous effect, and constitutional changes of concanavalin A due to exchanges of the incorporated metal ions may account for the observed viscosity increase. The study has demonstrated the potential of the MEMS sensor to determine sensitive viscosity changes within very small sample volumes, which could be of interest for various biotechnological applications.

Place, publisher, year, edition, pages
MDPI AG , 2017. Vol. 18, no 6
Keyword [en]
bioprocess; affinity assay; viscometer; glucose monitoring; concanavalin A; bacterial
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-30035DOI: 10.3390/ijms18061235Scopus ID: 2-s2.0-85020484678OAI: oai:DiVA.org:ri-30035DiVA: diva2:1119644
Available from: 2017-07-04 Created: 2017-07-04 Last updated: 2017-07-04Bibliographically approved

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