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Cellular transcriptional response to zirconia-based implant materials
University Medical Center Freiburg, Freiburg, Germany ; .
University Medical Center Freiburg, Freiburg, Germany ; .
University Medical Center Freiburg, Freiburg, Germany.
University Medical Center Freiburg, Freiburg, Germany.
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2017 (English)In: Dental Materials, ISSN 0109-5641, E-ISSN 1879-0097, Vol. 33, no 2, 241-255 p.Article in journal (Refereed) Published
Abstract [en]

Objective To adequately address clinically important issues such as osseointegration and soft tissue integration, we screened for the direct biological cell response by culturing human osteoblasts and gingival fibroblasts on novel zirconia-based dental implant biomaterials and subjecting them to transcriptional analysis. Methods Biomaterials used for osteoblasts involved micro-roughened surfaces made of a new type of ceria-stabilized zirconia composite with two different topographies, zirconium dioxide, and yttria-stabilized zirconia (control). For fibroblasts smooth ceria- and yttria-stabilized zirconia surface were used. The expression of 90 issue-relevant genes was determined on mRNA transcription level by real-time PCR Array technology after growth periods of 1 and 7 days. Results Generally, modulation of gene transcription exhibited a dual dependence, first by time and second by the biomaterial, whereas biomaterial-triggered changes were predominantly caused by the biomaterials’ chemistry rather than surface topography. Per se, modulated genes assigned to regenerative tissue processes such as fracture healing and wound healing and in detail included colony stimulating factors (CSF2 and CSF3), growth factors, which regulate bone matrix properties (e.g. BMP3 and TGFB1), osteogenic BMPs (BMP2/4/6/7) and transcription factors (RUNX2 and SP7), matrix collagens and osteocalcin, laminins as well as integrin ß1 and MMP-2. Significance With respect to the biomaterials under study, the screening showed that a new zirconia-based composite stabilized with ceria may be promising to provide clinically desired periodontal tissue integration. Moreover, by detecting biomarkers modulated in a time- and/or biomaterial-dependent manner, we identified candidate genes for the targeted analysis of cell-implant bioresponse during biomaterial research and development.

Place, publisher, year, edition, pages
2017. Vol. 33, no 2, 241-255 p.
Keyword [en]
Alveolar bone osteoblasts, Gingiva fibroblasts, Gene profiling, Zirconia composite, Dental implants
National Category
Materials Engineering Medical Engineering
Identifiers
URN: urn:nbn:se:ri:diva-29892DOI: 10.1016/j.dental.2016.12.005OAI: oai:DiVA.org:ri-29892DiVA: diva2:1109975
Available from: 2017-06-15 Created: 2017-06-15 Last updated: 2017-06-15Bibliographically approved

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Publisher's full texthttp://www.sciencedirect.com/science/article/pii/S0109564116307588

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