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Self-Assembly of Recombinant Silk as a Strategy for ChemicalFree Formation of Bioactive Coatings – a Real-Time Study
KTH, Stockholm, Sweden.
RISE - Research Institutes of Sweden, Bioscience and Materials, Chemistry, Materials and Surfaces.
KTH, Stockholm, Sweden.
KTH, Stockholm, Sweden.
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2017 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 18, no 3, 846-854 p.Article in journal (Refereed) Published
Abstract [en]

Functionalization of biomaterials with biologically active peptides can improve their performance after implantation. By genetic fusion to self-assembling proteins, the functional peptides can easily be presented on different physical formats. Herein, a chemical-free coating method based on self-assembly of the recombinant spider silk protein 4RepCT is described and used to prepare functional coatings on various biomaterial surfaces. The silk assembly was studied in real-time, revealing occurrence of continuous assembly of silk proteins onto surfaces and formation of nanofibrillar structures. The adsorbed amounts and viscoelastic properties were evaluated, and the coatings were shown to be stable against wash with hydrogen chloride, sodium hydroxide, and ethanol. Titanium, stainless steel, and hydroxyapatite were coated with silk fused to an antimicrobial peptide or a motif from fibronectin. Human primary cells cultured on the functional silk coatings show good cell viability and proliferation, implying potential to improve implant performance and acceptance by the body.

Place, publisher, year, edition, pages
2017. Vol. 18, no 3, 846-854 p.
Keyword [en]
coatings, recombinant spider silk, self-assembly, functionalization, implants
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:ri:diva-28252DOI: 10.1021/acs.biomac.6b01721OAI: oai:DiVA.org:ri-28252DiVA: diva2:1075668
Available from: 2017-02-20 Created: 2017-02-20 Last updated: 2017-03-31Bibliographically approved

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