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Significantly accelerated wound healing of full-thickness skin using a novel composite gel of porcine acellular dermal matrix and human peripheral blood cells
University of Gothenburg, Gothenburg, Sweden.
University of Gothenburg, Gothenburg, Sweden.
RISE - Research Institutes of Sweden, Bioscience and Materials, Chemistry, Materials and Surfaces.
TATAA Biocenter, Gothenburg, Sweden.
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2017 (English)In: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 26, no 2, 293-307 p.Article in journal (Refereed) Published
Abstract [en]

Herein, we report the fabrication of a novel composite gel from decellularized gal-gal-knockout porcine skin and human peripheral blood mononuclear cells (hPBMC) for full-thickness skin wound healing. Decellularized skin extracellular matrix (ECM) powder was prepared via chemical treatment, freeze-drying and homogenization. The powder was mixed with culture medium containing hyaluronic acid to generate a pig skin gel (PSG). The effect of the gel in regeneration of full-thickness wound was studied in nude mice. We found significantly accelerated wound closure already on day 15 in animals treated with PSG only or PSG+hPBMC as compared to untreated and hyaluronic acid treated controls (p<0.05). Addition of the hPBMC to the gel resulted in marked increase of host blood vessels as well as the presence of human blood vessels. At day 25, histologically, the wounds in animals treated with PSG only or PSG+hPBMC were completely closed as compared to controls. Thus, the gel facilitated generation of new skin with well arranged epidermal cells and restored bilayer structure of the epidermis and dermis. These results suggest that porcine skin ECM gel together with human cells may be a novel and promising biomaterial for medical applications especially for patients with acute and chronic skin wounds.

Place, publisher, year, edition, pages
Cognizant Communication Corporation, 2017. Vol. 26, no 2, 293-307 p.
National Category
Pharmacology and Toxicology Pharmaceutical Sciences Biomaterials Science
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URN: urn:nbn:se:ri:diva-27768DOI: 10.3727/096368916X692690PubMedID: 27503828OAI: oai:DiVA.org:ri-27768DiVA: diva2:1062571
Available from: 2017-01-06 Created: 2017-01-06 Last updated: 2017-04-26Bibliographically approved

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CiteExportLink to record
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