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Screening of nerve agent markers with hollow fiber-chemosorption of phosphonic acids
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Processum.
2016 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 1033-1034, 97-105 p.Article in journal (Refereed) Published
Abstract [en]

This report describes a method developed for extracting nerve gas markers such as phosphonic acids from urine and other aqueous samples. It involves single-step microextraction with chemosorption to hollow fibers that have been pre-soaked in a solution containing a derivatization reagent (3,5 triflouro methyl benzene diazomethane). The derivatives it forms with phosphonic acids can be sensitively detected by mass spectrometric detectors operating in negative chemical ionization (NCI) mode. Limits of quantification obtained in analyses of water and urine extracts by GC/MS in negative chemical ionization and selected ion monitoring mode were 0.1–10 and 0.5–10 ng/mL, respectively. Pentaflourophenyl diazomethane can also be used as a derivatization reagent, and the micro-extracts (which generate low background signals) can be sensitively analyzed by GC–MS/MS in NCI selected reaction monitoring (SRM) mode, using two specific transitions for both reagents. Thus, this sensitive approach can be flexibly modified to obtain confirmatory information, or address potential problems caused by interferences in some samples. © 2016 Elsevier B.V.

Place, publisher, year, edition, pages
2016. Vol. 1033-1034, 97-105 p.
Keyword [en]
Derivatization, GC/MS, Hollow fiber, Microextraction, Nerve gas markers, Phosphonic acids, Urine, Body fluids, Chemical analysis, Chemical reactions, Chemisorption, Ionization, Ionization of gases, Ionization of liquids, Mass spectrometry, Organic acids, Derivatizations, Nerve gas, Extraction
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-27600DOI: 10.1016/j.jchromb.2016.08.017Scopus ID: 2-s2.0-84982144028OAI: oai:DiVA.org:ri-27600DiVA: diva2:1059692
Note

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Available from: 2016-12-22 Created: 2016-12-21 Last updated: 2016-12-22Bibliographically approved

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