Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Imaging of amyloid-β in alzheimer’s disease transgenic mouse brains with ToF-SIMS using immunoliposomes
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor, Medicinteknik. Department of Physics, Chalmers University of Technology.
Show others and affiliations
2016 (English)In: Biointerphases, ISSN 1934-8630, E-ISSN 1559-4106, Vol. 11, no 2, 1-11 p., 02A312Article in journal (Refereed) Published
Abstract [en]

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been proven to successfully image different kinds of molecules, especially a variety of lipids, in biological samples. Proteins, however, are difficult to detect as specific entities with this method due to extensive fragmentation. To circumvent this issue, the authors present in this work a method developed for detection of proteins using antibody-conjugated liposomes, so called immunoliposomes, which are able to bind to the specific protein of interest. In combination with the capability of ToF-SIMS to detect native lipids in tissue samples, this method opens up the opportunity to analyze many different biomolecules, both lipids and proteins, at the same time, with high spatial resolution. The method has been applied to detect and image the distribution of amyloid-β (Aβ), a biologically relevant peptide in Alzheimer’s disease (AD), in transgenic mouse brain tissue. To ensure specific binding, the immunoliposome binding was verified on a model surface using quartz crystal microbalance with dissipation monitoring. The immunoliposome binding was also investigated on tissue sections with fluorescence microscopy, and compared with conventional immunohistochemistry using primary and secondary antibodies, demonstrating specific binding to Aβ. Using ToF-SIMS imaging, several endogenous lipids, such as cholesterol and sulfatides, were also detected in parallel with the immunoliposome-labeled Aβ deposits, which is an advantage compared to fluorescence microscopy. This method can thus potentially provide further information about lipid–protein interactions, which is important to understand the mechanisms of neurodegeneration in AD.

Place, publisher, year, edition, pages
American Institute of Physics (AIP), 2016. Vol. 11, no 2, 1-11 p., 02A312
National Category
Biophysics Biochemistry and Molecular Biology Analytical Chemistry
Identifiers
URN: urn:nbn:se:ri:diva-27648DOI: 10.1116/1.4940215Scopus ID: 2-s2.0-85000866221OAI: oai:DiVA.org:ri-27648DiVA: diva2:1059498
Available from: 2016-12-22 Created: 2016-12-21 Last updated: 2017-01-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopusOpen access article at publishers website
By organisation
Medicinteknik
In the same journal
Biointerphases
BiophysicsBiochemistry and Molecular BiologyAnalytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 7 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
v. 2.26.0