A new design for permanent, low-cost, and planar fluidic channels on TiO2 nanoparticle coated paperboard is demonstrated. Initially superhydrophobic TiO2 nanoparticle coatings can be converted to hydrophilic by ultraviolet (UVA) light, and fluidic channels can be generated. A simple water treatment after the UVA illumination converts the channels permanent when nanoparticles are removed from the illuminated and wetted areas as shown by water contact angle, FE-SEM, XPS, and ToF-SIMS analysis. This suggests new routes for inexpensive, easy to use point-of-care diagnostics based on planar fluidic channels.