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Recellularization of acellular human small intestine using bone-marrow stem cells
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
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2013 (English)In: Stem Cells Translational Medicine, ISSN 2157-6564, E-ISSN 2157-6580, Vol. 2, no 4, 307-315 p.Article in journal (Refereed)
Abstract [en]

We aimed to produce an acellular human tissue scaffold with a view to test the possibility of recellularization with bone marrow stem cells to produce a tissue-engineered small intestine (TESI). Human small-bowel specimens (n = 5) were obtained from cadaveric organ donors and treated sequentially with 6% dimethyl sulfoxide in hypotonic buffer, 1% Triton X-100, and DNase. Each small intestine (SI) piece (6 cm) was recellularized with EPCAM+ and CD133+ allogeneic bone marrow stem cells. Histological and molecular analysis demonstrated that after decellularization, all cellular components and nuclear material were removed. Our analysis also showed that the decellularized human SI tissue retained its histoarchitecture with intact villi and major structural proteins. Protein films of common extracellular matrix constituents (collagen I, laminin, and fibronectin) were found in abundance. Furthermore, several residual angiogenic factors were found in the decellularized SI. Following recellularization, we found viable mucin-positive goblet cells, CK18+ epithelial cells in villi adjacent to a muscularis mucosa with α-actin+ smooth muscle cells, and a high repopulation of blood vessels with CD31+ endothelial cells. Our results show that in the future, such a TESI would be ideal for clinical purposes, because it can be derived from the recipient's own immunocompatible bone marrow cells, thus avoiding the use of immunosuppression.

Place, publisher, year, edition, pages
2013. Vol. 2, no 4, 307-315 p.
Keyword [en]
Recellularization of acellular human small intestine using bone-marrow stem cells
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-27491DOI: 10.5966/sctm.2012-0108OAI: oai:DiVA.org:ri-27491DiVA: diva2:1054496
Note
A3088.Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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CiteExportLink to record
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