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Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect
YKI – Ytkemiska institutet.
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2003 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 100, 6331-6336 p.Article in journal (Refereed)
Abstract [en]

The assessment of biomaterial compatibility relies heavily on the analysis of macroscopic cellular responses to material interaction. However, new technologies have become available that permit a more profound understanding of the molecular basis of cell-biomaterial interaction. Here, both conventional phenotypic and contemporary transcriptomic (DNA microarray-based) analysis techniques were combined to examine the interaction of cells with a homologous series of copolymer films that subtly vary in terms of surface hydrophobicity. More specifically, we used differing combinations of N-isopropylacrylamide, which is presently used as an adaptive cell culture substrate, and the more hydrophobic, yet structurally similar, monomer N-tert-butylacrylamide. We show here that even discrete modifications with respect to the physiochemistry of soft amorphous materials can lead to significant impacts on the phenotype of interacting cells. Furthermore, we have elucidated putative links between phenotypic responses to cell-biomaterial interaction and global gene expression profile alterations. This case study indicates that high-throughput analysis of gene expression not only can greatly refine our knowledge of cell-biomaterial interaction, but also can yield novel biomarkers for potential use in biocompatibility assessment

Place, publisher, year, edition, pages
2003. Vol. 100, 6331-6336 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-27387OAI: oai:DiVA.org:ri-27387DiVA: diva2:1054391
Note
A1622Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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