Protein-surfactant interactions play a key role in competitive adsorption and desorption of adsorbed proteins by surfactants. The influence of nonmicellar solutions of an anionic surfactant, sodium dodecane-sulfonate, on the interaction forces between preadsorbed layers of Iysozyme on mica were investigated using the surface force apparatus. In the absence of surfactant, Iysozyme adsorbs irreversibly and neutralizes the negative mica surface charge. The force profile indicates that the adsorbed layer is heterogeneous and deformable, consisting of protein monomers and loosely associated dimers. An adhesion force is established upon contact of the adsorbed layers. Surfactant binding increases the interfacial charge and eliminates the adhesion force between opposing surfaces. Large-scale desorption or conforma-tional change in response to surfactant binding is not observed, although partial desorption of the outer members of adsorbed dimers is induced at the highest surfactant concentration investigated.