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Liposomal surface potential and bilayer packing as affected by PEG-lipid inclusion
YKI – Ytkemiska institutet.
2000 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 16, 3696-3702 p.Article in journal (Refereed)
Abstract [en]

The surface potentials and the packing properties of phospholipid liposomes containing anionic lipids with covalently attached poly(ethylene glycol) polymers (PEG(2000)-PE) were investigated. The negative surface potential induced by the PEG-lipid in EPC/cholesterol and SPC/cholesterol liposomes was determined by means of titration of the fluorescent probe 4-heptadecyl-7-hydroxycoumarin (HC). The shift in the apparent pK of the probe corresponds to an induced potential of -13 mV for 5 mol % PEG-lipid in the phospholipid/cholesterol (ionic strength = 150 mM). For EPC liposomes without cholesterol, 5 mol % PEG-lipid did not cause any detectable shift in the pK of the probe. This indicates that the EPC liposome surface in fact carries a net negative charge. The above results were supported by determinations of the potential. The packing order of the outer and inner parts of the phospholipid/cholesterol bilayer membranes was investigated by fluorescence depolarization anisotropy of the probes HC and 1,6-diphenyl-1,3,5-hexatriene (DPH). The PEG-lipid inclusion caused an increase in the anisotropy of HC, located at the water/hydrocarbon interface. In addition, a small increase in anisotropy was observed for DPH upon PEG-lipid inclusion. Since DPH is situated in the hydrocarbon region, these data together with the HC data indicate that the PEG-lipids impose restrictions on the molecular motion in the outer as well as the inner parts of the hydrocarbon region.

Place, publisher, year, edition, pages
2000. Vol. 16, 3696-3702 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-27228OAI: oai:DiVA.org:ri-27228DiVA: diva2:1054232
Note
A1369Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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