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Interaction forces between BSA layers adsorbed on silica surfaces measured with an atomic force microscope
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2004 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 108, 5365-5371 p.Article in journal (Refereed)
Abstract [en]

The interaction forces between bovine serum albumin (BSA) layers adsorbed on silica surfaces have been measured using an atomic force microscope (AFM) in conjunction with the colloid probe technique. Measurements of force-distance curves were made at different pH values and electrolyte concentrations (NaCl and CaCl2). The interaction at long range is dominated by electrical double-layer forces, while at short surface separations an additional repulsion due to the compression of the adsorbed protein layers appears. However, prior to this steric interaction, when the pH is above the isoelectric point of the protein and at high salt concentration, a non-DLVO repulsive interaction is observed. This behavior is explained if the presence of hydration forces in the system is assumed. Theoretical predictions including a hydration term in the DLVO theory fit the experimental results satisfactorily. The results presented in this article provide a direct confirmation that the AFM colloid probe technique can provide a useful way of directly quantifying the interaction of biological macromolecules.

Place, publisher, year, edition, pages
2004. Vol. 108, 5365-5371 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26936OAI: oai:DiVA.org:ri-26936DiVA: diva2:1053939
Note
A1740Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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YKI – Ytkemiska institutet
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