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Interactions between poly(ethylene oxide) coated surfaces and between such surfaces and proteins
YKI – Ytkemiska institutet.
YKI – Ytkemiska institutet.
1998 (English)In: Journal of Dispersion Science and Technology, ISSN 0193-2691, E-ISSN 1532-2351, Vol. 19, 1107-1126 p.Article in journal (Refereed)
Abstract [en]

Surfaces coated with poly(ethylene oxide) containing nonionic polymers are of interest in medical applications due to, among other things, the low adsorption of proteins on such surfaces. In this paper we have studied the interfacial properties of surfaces coated with PEO by measuring the forces acting between two such surfaces in water and across a protein solution as well as between one such surface and a surface carrying adsorbed proteins. One type of surface coating was a graft copolymer of poly(ethylene imine) and poly(ethylene oxide) where the cationic poly(ethylene imine) group anchored the polymer to negatively charged mica surfaces. Three different ways to prepare this coating was used and compared. It was found that this coating was not stable in the presence of lysozyme, a small positively charged protein, when the PEO graft density was low. The other type of coating was obtained by adsorbing ethyl(hydroxyethyl)-cellulose onto hydrophobised mica surfaces. The driving force for adsorption is in this case the hydrophobic interaction between nonpolar segments of the polymer and the surface. The EHEC coating was stable in the presence of lysozyme and the interactions between adsorbed layers of lysozyme and EHEC coated surfaces are purely repulsive due to long-range steric forces.

Place, publisher, year, edition, pages
1998. Vol. 19, 1107-1126 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26867OAI: oai:DiVA.org:ri-26867DiVA: diva2:1053870
Note
A1161Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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