Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effects of amino acid composition on protein adsorption
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, YKI – Ytkemiska institutet.
1996 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 178, p. 160-167Article in journal (Refereed)
Abstract [en]

The adsorption at silica and methylated silica of genetically engineered derivatives of Z and its dimer ZZ, where Z is a hydrophilic synthetic IgG binding domain derived from staphylococcal protein A, was studied with in situ ellipsometry. The protein modifications consisted of introducing short peptide stretches near the C-terminus of the protein. Using this approach, oligopeptide stretches containing hydrophobic tryptophan (Trp) or isoleucine (Ile) [(AlaTrpTrpPro)n or (AlaIleIlePro)n (0≤n≤2), denoted Tn and In, respectively] were introduced in the protein. For comparison, the adsorption of the inserted peptide stretches (Tn and In), as well as of Trp and Ile oligomers, was investigated as well. Increasing the number of Trp residues resulted in an increased adsorption for both ZZTn, ZTn, Tn, and Trpn. At a given number of Trp residues, the adsorbed amount of the ZZ derivatives is larger than that of the peptides, but about the same as that of the Z proteins. Analogous although somewhat smaller effects were obtained for the Ile-derivatized proteins. These results are discussed in terms of the "block copolymer" nature of the proteins. Theoretical calculations using a mean-field lattice model for block copolymer adsorption gave a qualitative agreement with the experimentally obtained results.

Place, publisher, year, edition, pages
1996. Vol. 178, p. 160-167
Keywords [en]
Adsorption, amino acid, ellipsometry, peptide, protein
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26754OAI: oai:DiVA.org:ri-26754DiVA, id: diva2:1053757
Note
A1058Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2020-12-01Bibliographically approved

Open Access in DiVA

No full text in DiVA

By organisation
YKI – Ytkemiska institutet
In the same journal
Journal of Colloid and Interface Science
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 41 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf