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Structure of protein layers during competitive adsorption
YKI – Ytkemiska institutet.
1996 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 180, 339-349 p.Article in journal (Refereed)
Abstract [en]

The formation of protein layers during competitive adsorption was studied with ellipsometry. Single, binary, and ternary protein solutions of human serum albumin (HSA), IgG, and fibrinogen (Fgn) were investigated at concerntrations corresponding to blood plasma diluted 1/100. As a model surface, hydrophobic hexamethyldisiloxane (HMDSO) plasma polymer modified silica was used. By using multiambient media measurements of the bare substrate prior to protein adsorption the adsorbed amount as well as the thickness and refractive index of the adsorbed protein layer could be followed in situ and in real time. Under conditions used in these experiments neither IgG nor fibrinogen could fullydisplace serum albumin from the interface. The buildup of the protein layer occured via different mechanisms for the different protein systems. Fgn adsorbed in a rather flat orientation at low adsorbed amounts, while at higher surface coverage the protein reoriented to a more upright orientation in order to accommodate more molecules in the adsorbed layer. IgG adsorption proceeded mainly end on with little reorientation or conformational change on adsorption. Finally, for HSA an adsorbed layer thickness greater than the molecular dimensions was observed at high concentrations ( although not at low ), indicating that aggregates or multilayers formed on HMDSO plasma polymer surfaces. For all protein mixtures the adsorbed layer structure and buildup indicated that Fgn was the protein dominating the adsorbed layer, although HSA partially blocked the adsorption of this protein. At high surface concentration, HSA/Fgn mixtures show an abrupt change in both adsorbed layer thickness and refractive index suggesting, e.g., an interfacial phase transition of the mixed protein layer. A similar but less pronounced behavior was observed for HSA/IgG. For IgG/Fgn and HSA/Fgn a buildup of the adsorbed layer similar to that displayed by Fgn alone was observed.

Place, publisher, year, edition, pages
1996. Vol. 180, 339-349 p.
Keyword [en]
Adsorption, competitive adsorption, ellipsometry, plasma polymer, proteins
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26748OAI: oai:DiVA.org:ri-26748DiVA: diva2:1053751
Note
A1037Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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