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Sequential adsorption of human serum albumin (HSA), immunoglobulin G (IgG), and fibrinogen (Fgn) at HMDSO plasma polymer surfaces
YKI – Ytkemiska institutet.
1997 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 193, 88-95 p.Article in journal (Refereed)
Abstract [en]

The sequential adsorption of human serum albumin (HSA), immunoglobulin G (IgG), and fibrinogen (Fgn) at hexamethyldisiloxane (HMDSO) plasma polymer surfaces was investigated with ellipsometry and total internal reflectance fluorescence spectroscopy (TIRF) as a function of adsorption time, pH, and excess electrolyte concentration. HSA was found to self-exchange very slowly (≈hours) at pH 7.2, irrespective of adsorption time in the range 90 seconds to 90 minutes. Preadsorbed HSA was exchanged by Fgn and IgG only to a limited extent irrespectively of pH (5≤pH≤8) and excess electrolyte concentration (5 mM≤Cs≤150 mM). At an excess electrolyte concentration of 150 mM, the sequential adsorption of Fgn and IgG was dramatically reduced by HSA preadsorption, irrespective of pH. At an excess electrolyte concentration of 5 mM, on the other hand, there were indications of second layer adsorption of Fgn and IgG.

Place, publisher, year, edition, pages
1997. Vol. 193, 88-95 p.
Keyword [en]
Adsorption, ellipsometry, plasma polymers, proteins, TIRF
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26737OAI: oai:DiVA.org:ri-26737DiVA: diva2:1053740
Note
A1127Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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