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Sequential adsorption of human serum albumin (HSA), immunoglobulin G (IgG), and fibrinogen (Fgn) at HMDSO plasma polymer surfaces
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, YKI – Ytkemiska institutet.
RISE Research Institutes of Sweden, Bioeconomy and Health, Chemical Process and Pharmaceutical Development.ORCID iD: 0009-0001-5651-8461
1997 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 193, p. 88-95Article in journal (Refereed) Published
Abstract [en]

The sequential adsorption of human serum albumin (HSA), immunoglobulin G (IgG), and fibrinogen (Fgn) at hexamethyldisiloxane (HMDSO) plasma polymer surfaces was investigated with ellipsometry and total internal reflectance fluorescence spectroscopy (TIRF) as a function of adsorption time, pH, and excess electrolyte concentration. HSA was found to self-exchange very slowly (≈hours) at pH 7.2, irrespective of adsorption time in the range 90 seconds to 90 minutes. Preadsorbed HSA was exchanged by Fgn and IgG only to a limited extent irrespectively of pH (5≤pH≤8) and excess electrolyte concentration (5 mM≤Cs≤150 mM). At an excess electrolyte concentration of 150 mM, the sequential adsorption of Fgn and IgG was dramatically reduced by HSA preadsorption, irrespective of pH. At an excess electrolyte concentration of 5 mM, on the other hand, there were indications of second layer adsorption of Fgn and IgG.

Place, publisher, year, edition, pages
1997. Vol. 193, p. 88-95
Keywords [en]
Adsorption, ellipsometry, plasma polymers, proteins, TIRF
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26737OAI: oai:DiVA.org:ri-26737DiVA, id: diva2:1053740
Note

A1127

Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2024-06-25Bibliographically approved

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Lassen, Bo

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