Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A fluorescence method for quantitative measurements of specific protein at powder surfaces
YKI – Ytkemiska institutet.
1999 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 12, p. 429-440Article in journal (Refereed)
Abstract [en]

A new method using fluorescence labeled proteins was developed to determine the quantitative amount of specific protein at the powder surface. The method is based on steady-state fluorescence measurements of pyrene labeled proteins with oxygen gas phase quenching at the powder surface. The surface load of protein was measured for spray-dried dextran powders containing bovine serum albumin (BSA). The results show a patchwise surface load of about 1.3 mg/m2 at a concentration of 0.33 % (dry weight) BSA in the powder. The patchwise surface load stays constant with increased BSA concentration in the powder.

Place, publisher, year, edition, pages
1999. Vol. 12, p. 429-440
Keywords [en]
Fluorescence quenching, pyrene, bovine serum albumin, spray-drying, protein adsorption
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26491OAI: oai:DiVA.org:ri-26491DiVA, id: diva2:1053493
Note
A1245Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2017-11-29Bibliographically approved

Open Access in DiVA

No full text in DiVA

By organisation
YKI – Ytkemiska institutet
In the same journal
Colloids and Surfaces B: Biointerfaces
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 10 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
v. 2.35.3