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Microemulsions as reaction media for immobilization proteins to hydrophilized surfaces
Berol Nobel, Sweden.
YKI – Ytkemiska institutet. Berol Nobel, Sweden.
1992 (English)In: Colloids and Surfaces, ISSN 0166-6622, E-ISSN 1873-4340, Vol. 63, p. 273-280Article in journal (Refereed)
Abstract [en]

The effect of microemulsion as reaction medium for immobilization of proteins to polystyrene hydrophilized with poly(ethylene glycol) or polysaccharide has been investigated. The amount of albumin, immunoglobulin G (IgG) and collagen immobilized from microemulsion was increased 10-100 times, compared with immobilization from an aqueous buffer system. The stability of anti-IgG and interleukin-2 was studied in different microemulsion systems. Anti-IgG was found to be stable in microemulsions based on sodium bis(2-ethylhexyl)sulphosuccinate (AOT) and C12EO5, whereas the non-ionic surfactant di-C9EO9 almost instantly destroyed the antigen-binding properties. Interleukin-2 completely lost its biological activity in an AOT-based microemulsion. We have also found that the choice of microemulsion influences coupling efficiency of the protein. An AOT-based system is preferred for immobilization of mycoplasma antibody, while a microemulsion based on C12EO5 is preferred for borrelia antigen.

Place, publisher, year, edition, pages
1992. Vol. 63, p. 273-280
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26394OAI: oai:DiVA.org:ri-26394DiVA, id: diva2:1053396
Note
A783Available from: 2016-12-08 Created: 2016-12-08 Last updated: 2017-11-29Bibliographically approved

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