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Adsorption of lysozyme, beta-casein and their layer-by-layer formation on hydrophilic surfaces: Effect of ionic strength
YKI – Ytkemiska institutet.
YKI – Ytkemiska institutet.
YKI – Ytkemiska institutet.
2010 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 77, no 1, 1-11 p.Article in journal (Refereed)
Abstract [en]

The adsorbed amount and layer structure of lysozyme, _x0002_-casein and mixed layers of the two proteins were studied on hydrophilic silica and quartz surfaces using the following techniques: ellipsometry, quartz crystal microbalance with dissipation monitoring (QCM-D) and total internal reflection fluorescence (TIRF). Particular emphasis was put on the effect of solution ionic strength on the layer formation. Both lysozyme and _x0002_-casein showed a higher affinity for the silica surface when adsorbed from a solution of low ionic strength even though _x0002_-casein and silica are negatively charged at the pH used. No _x0002_-casein remained adsorbed after rinsing with a 150mMbuffer solution. The adsorbed amount of lysozyme on silica exceeded a monolayer coverage irrespective of the solution conditions and displayed a rigid structure. _x0002_-Casein forms more than a single layer on pre-adsorbed lysozyme; an inner flat layer and an outer layer with an extended structure, which largely desorbs on rinsing. The build-up through sequential adsorption of lysozyme and _x0002_-casein is favoured at intermediate and high ionic strength. The total adsorbed amount increased slightly with each deposition cycle and the mixed lysozyme/_x0002_-casein layers contain higher amounts of protein compared to those of pure lysozyme or _x0002_-casein. Sequential adsorption gives rise to a proteinaceous layer consisting of both lysozyme and _x0002_-casein. The protein layers are probably highly interpenetrated with no clear separation between them.

Place, publisher, year, edition, pages
2010. Vol. 77, no 1, 1-11 p.
Keyword [en]
Adsorption of lysozyme, beta-casein and their layer-by-layer formation on hydrophilic surfaces: Effect of ionic strength, Lysozyme, [Beta- ]Casein, Layer-by-layer, Protein adsorption, Multilayers, Ellipsometry, QCM-D, TIRF, Solvent content
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26387DOI: 10.1016/j.colsurfb.2009.12.019OAI: oai:DiVA.org:ri-26387DiVA: diva2:1053389
Note
A2068Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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CiteExportLink to record
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Citation style
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