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Correlation of the adhesive properties of cells to N-iso-propylacrylamide/N-tert-butylacrylamide copolymer surfaces with changes in the surface structure using contact angle measurements, molecular simulations and Raman spectroscopy
YKI – Ytkemiska institutet.
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2005 (English)In: Chemistry of Materials, ISSN 0897-4756, E-ISSN 1520-5002, Vol. 17, 3889-3898 p.Article in journal (Refereed)
Abstract [en]

A series of copolymers of N-isopropylacrylamide (NIPAM) and the more hydrophobic comonomer N-tert-butylacrylamide (NTBAM), with increasing NTBAM content (i.e. increasing hydrophobicity) were prepared. The adhesion of human endothelial cells on polymer films prepared from copolymers of NIPAM:NTBAM was observed to increase with increasing polymer hydrophobicity. However, in the absence of serum, cell adhesion to the different surfaces was statistically indistinguishable. Thus, it appears that the copolymer films differentially support cell adhesion due to selective adsorption of proteins from the physiological environment (the serum). Using contact angle measurements, molecular simulations and Raman spectroscopy to characterize the different surfaces, we show evidence that the different behavior of the films of increasing hydrophobicity is actually due to the different chemical properties of the surfaces with increasing content of NTBAM in the copolymers. As the NTBAM content is increased, the number of NH residues at the surface decreases, due to the additional steric hindrance of the bulkier NTBAM group, which results in decreased hydrogen bonding and thus decreased adsorption of proteins such as albumin. However, in some cases, the adsorption is driven by hydrophobic interactions, and proteins such as fibronectin were found to adsorb more to the films with a higher content of NTBAM. There appears, thus, to be a direct correlation between surface composition and protein binding and the subsequent cell adhesion.

Place, publisher, year, edition, pages
2005. Vol. 17, 3889-3898 p.
Keyword [en]
N-isopropylacrylamide, N-tert-butylacrylamide, cell adhesion, protein binding, contact angle, surface energy, hydrophobicity, Raman
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26346OAI: oai:DiVA.org:ri-26346DiVA: diva2:1053348
Note
A1712Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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