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An alternative approach to decellularize whole porcine heart
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.
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2014 (English)In: BioResearch Open Access, ISSN 2164-7844, E-ISSN 2164-7860, Vol. 3, no 6, 327-338 p.Article in journal (Refereed)
Abstract [en]

Scaffold characteristics are decisive for repopulating the acellular tissue with cells. A method to produce such a scaffold from intact organ requires a customized decellularization protocol. Here, we have decellularized whole, intact porcine hearts by serial perfusion and agitation of hypotonic solution, an ionic detergent (4% sodium deoxycholate), and a nonionic detergent (1% Triton X-100). The resultant matrix was characterized for its degree of decellularization, morphological and functional integrity. The protocol used resulted in extensive decellularization of the cardiac tissue, but the cytoskeletal elements (contractile apparatus) of cardiomyocytes remained largely unaffected by the procedure although their membranous organelles were completely absent. Further, several residual angiogenic growth factors were found to be present in the decellularized tissue.

Place, publisher, year, edition, pages
2014. Vol. 3, no 6, 327-338 p.
Keyword [en]
heart, ECM (extracellular matrix), growth factors, angiogenesis, vasculogenesis, An alternative approach to decellularize whole porcine heart
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-26330DOI: 10.1089/biores.2014.0046OAI: oai:DiVA.org:ri-26330DiVA: diva2:1053332
Note
A3390Available from: 2016-12-08 Created: 2016-12-08Bibliographically approved

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