This study focused on wood density and annual ring width in Norway spruce (Picea abies (L.) Karst.) grown in unevenaged stands (UAS). In total, 96 trees were harvested from five UAS that had been managed by single-tree selection for decades. A data set of 27 trees from even-aged stands (EAS) was used for comparison. In the UAS trees, high density and narrow annual rings were found in the juvenile wood near the pith. Thereafter, wood density rapidly decreased until the 20th annual ring, followed by an increase toward the bark. In the outermost rings, wood density again slightly decreased. The trends in wood density in the UAS trees correspond with those reported for naturally regenerated, even-aged Norway spruce stands, with the exception of the decrease in the outermost rings. A mixed linear model with ring width, cambial age, and canopy position as fixed parameters accounted for 53% of the variation in wood density of the UAS trees. In contrast to UAS trees, EAS trees showed increased wood density in the outer rings as a result of decreasing growth rate. The abrupt change in wood density of UAS trees may affect the properties of timber sawn close to the pith.