Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Aggregates in acidic solutions of chitosans detected by static laser light scattering
SIK – Institutet för livsmedelsforskning.
Show others and affiliations
1994 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 25, no 1, p. 13-23Article in journal (Refereed)
Abstract [en]

Chitosans having degrees of N-acetylation, FA, ranging from 0 to 0·6, were randomly degraded to different molecular weights and studied by multi angle static laser light scattering (LLS). Under the given experimental conditions, negative second virial coefficients of the solutions, A?2, revealed the presence of concentration dependent aggregates. Attempts to remove the aggregates, or to influence the aggregation behavior, were made by ultracentrifugation and extensive filtering of the solutions. Modification of the solvent conditions such as pH, ionic strength and temperature were carried out, and chitosan solutions were digested with an acidic proteinase. Non-degraded samples and chitosans prepared by both heterogeneous and homogeneous N-deacetylation of chitin were also studied. In all cases, the negative A?2 remained. However, it was observed that ultracentrifugation and filtering of the solutions decreased the measured molecular weights and radii of gyration, indicating that some of the material of high molecular weight and size could be removed by ultracentrifugation and filtration. The chemical nature of the physical basis of the molecular association was not revealed. Nevertheless, by the use of gel permeation chromatography coupled to an on-line low angle laser light scattering instrument and a differential refractive index concentration detector (HPSEC-LALLS-RI), a bimodal molecular weight distribution was observed in which about 5% of the sample had a very high molecular weight. These results coupled with the positive virial coefficients obtained earlier from osmotic pressure measurements suggest that a small fraction of the chitosan is aggregated to high molecular weight material, probably following a closed association model. Electron microscopy revealed the presence of some supramolecular structures. The positive second virial coefficients obtained earlier from osmometry are in harmony with these findings. The results demonstrate the occurrence of reversible aggregation in chitosan solutions. Static laser light scattering therefore cannot readily be used to determine molecular weights and sizes of chitosans under these conditions. It was not possible to correlate the extent of aggregation with the chemical composition of the chitosans. © 1994.

Place, publisher, year, edition, pages
1994. Vol. 25, no 1, p. 13-23
Keywords [en]
Food Engineering
Keywords [sv]
Livsmedelsteknik
National Category
Food Science
Identifiers
URN: urn:nbn:se:ri:diva-8434DOI: 10.1016/0144-8617(94)90157-0OAI: oai:DiVA.org:ri-8434DiVA, id: diva2:966306
Available from: 2016-09-08 Created: 2016-09-08 Last updated: 2017-11-21Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full texthttp://www.scopus.com/inward/record.url?eid=2-s2.0-0028728150&partnerID=40&md5=5c857f8267a9d950155b0e8ab39162ca
By organisation
SIK – Institutet för livsmedelsforskning
In the same journal
Carbohydrate Polymers
Food Science

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 61 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
v. 2.35.7