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Spatial distributions of lipids in atherosclerosis of human coronary arteries studied by time-of-flight secondary ion mass spectrometry
Wihuri Research Institute, Finland.
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Sveriges tekniska forskningsinstitut, SP – Sveriges Tekniska Forskningsinstitut / Funktionella material (KMf).ORCID iD: 0000-0002-2696-7215
University of Helsinki, Finland.
University of Helsinki, Finland.
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2015 (English)In: American Journal of Pathology, ISSN 0002-9440, E-ISSN 1525-2191, Vol. 185, no 5, p. 1216-1233Article in journal (Refereed) Published
Abstract [en]

The accurate spatial distribution of various lipid species during atherogenesis has remained unexplored. Herein, we used time-of-flight secondary ion mass spectrometry (TOF-SIMS) to analyze the lipid dis-tribution in human coronary artery cryosections. The images from the TOF-SIMS allowed visualization ofions derived from individual species of cholesterol esters, phospholipids, and triacylglycerols in thecontext of lesion characteristics and severity. In addition, cholesterol-containing crystal-like structureswere seen in high-resolution images of advanced lesions. The ratio of cholesterol fragment ions (m/z385:m/z 369) was found to differentiate unesterified cholesterol from cholesterol esters. This ratiochanged during atherogenesis and in different areas of the lesions, reflecting differences in theaccumulation of the two forms of cholesterol. Thus, atheromas were characterized by accumulation ofcholesterol esters with apolipoprotein B near the intima-media border, whereas in the complicatedlesions, unesterified cholesterol dominated in neovessel-containing areas enriched in glycophorin A.Interestingly, triacylglycerols were found in areas surrounding neovessels and lacking either form ofcholesterol. The lipid composition of the tunica media reflected the alterations observed in the intimallipids, yet being more subtle. The detailed molecular information obtained by TOF-SIMS revealedunanticipated differences in the type and composition of the accumulating lipids in different stages ofatherogenesis, notably the spatial segregation of cholesterol and triglycerides in the advancing lesions.

Place, publisher, year, edition, pages
2015. Vol. 185, no 5, p. 1216-1233
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Natural Sciences
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URN: urn:nbn:se:ri:diva-6811DOI: 10.1016/j.ajpath.2015.01.026PubMedID: 25805604Scopus ID: 2-s2.0-84928041221Local ID: 23605OAI: oai:DiVA.org:ri-6811DiVA, id: diva2:964651
Available from: 2016-09-08 Created: 2016-09-08 Last updated: 2023-06-05Bibliographically approved

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