Owners of stables and owners of zoos have difficulty finding an economically sustainable deposition of their produced manure. More than two million tons of horse manure are produced in urban environments in Sweden every year. If the manure cannot be used as fertilizer on farm land it is classified as a waste fraction and should be handled according to current regulations. The manure is a valuable waste fraction that contains both energy and nutrients. If the manure cannot be spread on farm land more applications need to be identified, where the energy and nutrients in the manure can be used. The focus in this study is to investigate possible applications for the usage of horse and zoo manure within Borås municipality where, among other things, a waste water treatment plant and a combined heat and power plant are available. Horse and zoo manure have been investigated in the following applications: co-digestion with sewage sludge at a waste water treatment plant (lab experiments), co-digestion with food waste (theoretical), co-incineration with waste (full scale) and co-incineration with biomass (theoretical). Potential quantity of manure and economical and legal aspects have been studied as well. There is no compilation of the number of horses in the country which makes it hard to estimate the true quantity of manure. The quantity of manure from the zoos are somewhat easier to estimate since the zoo owners are fewer and have knowledge of their manure production. The co-digestions experiments in this study showed that addition of horse manure to digestion can be of interest in many ways, among other things it can give a more stable biogas production and a possible decrease in the Cd/P-ratio in the end product. Horse manure turned out to have a faster degradation rate compared to zoo manure, however the degradation rate was lower than that of sewage sludge. Zoo manure gave a relatively low biogas production compared to horse manure at thermophilic conditions. The co-incineration trial with waste and manure gave no negative effect with regard to emissions and operation. However, the amount of manure added to the incineration trial was low. The theoretical studies regarding the co-incineration with biomass, showed two potential alternatives that need to be investigated further. Interesting aspects to look further upon, based on this study, are for example: • Laws and regulations in the EU regarding manure. • Co-digestion of manure and sewage sludge in a larger scale. • Laws and regulations and costs regarding incineration of manure with biomass.