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Enzymatic hydrolysis of Norway spruce and sugarcane bagasse after treatment with 1-allyl-3-methylimidazolium formate
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Processum.
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2013 (English)In: Journal of chemical technology and biotechnology (1986), ISSN 0268-2575, E-ISSN 1097-4660, Vol. 88, no 12, p. 2209-2215Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Enzymatic hydrolysis of cellulose in lignocellulosic materials suffers from slow reaction rates due to limited access to enzyme adsorption sites and to the high crystallinity of the cellulose. In this study, an attempt was made to facilitate enzymatic hydrolysis by pretreatment of cellulosic materials using the ionic liquid (IL) 1-allyl-3-methylimidazolium formate ([Amim][HCO2]) under mild reaction conditions. The effect of the IL was compared with that of thermochemical pretreatment under acidic conditions. RESULTS: The lignocellulosic substrates investigated were native and thermochemically pretreated Norway spruce and sugarcane bagasse. Microcrystalline cellulose (Avicel) was included for comparison. The IL treatments were performed in the temperature range 45-120°C and, after regeneration and washing of the cellulosic substrates, enzymatic saccharification was carried out at 45°C for 72h. After 12h of hydrolysis, the glucose yields from regenerated native spruce and sugarcane bagasse were up to nine times higher than for the corresponding untreated substrates. The results also show positive effects of pretreatment using [Amim][HCO2] on the hydrolysis of xylan and mannan. Conclusion: The present work demonstrates that both native wood and agricultural residues are readily soluble in [Amim][HCO2] under gentle conditions, and that pretreatment with ionic liquids such as [Amim][HCO2] warrants further attention as a potential alternative to conventional pretreatment techniques. © 2013 Society of Chemical Industry.

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2013. Vol. 88, no 12, p. 2209-2215
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Natural Sciences
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URN: urn:nbn:se:ri:diva-188DOI: 10.1002/jctb.4089OAI: oai:DiVA.org:ri-188DiVA, id: diva2:935972
Available from: 2016-06-13 Created: 2016-06-13 Last updated: 2017-11-28Bibliographically approved

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CiteExportLink to record
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