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Production of cellulosic ethanol and enzyme from waste fiber sludge using SSF, recycling of hydrolytic enzymes and yeast, and recombinant cellulase-producing Aspergillus niger
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Processum.
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2014 (English)In: Journal of Industrial Microbiology & Biotechnology, ISSN 1367-5435, E-ISSN 1476-5535, Vol. 41, no 8, p. 1191-1200Article in journal (Refereed) Published
Abstract [en]

Bioethanol and enzymes were produced from fiber sludges through sequential microbial cultivations. After a first simultaneous saccharification and fermentation (SSF) with yeast, the bioethanol concentrations of sulfate and sulfite fiber sludges were 45.6 and 64.7 g/L, respectively. The second SSF, which included fresh fiber sludges and recycled yeast and enzymes from the first SSF, resulted in ethanol concentrations of 38.3 g/L for sulfate fiber sludge and 24.4 g/L for sulfite fiber sludge. Aspergillus niger carrying the endoglucanase-encoding Cel7B gene of Trichoderma reesei was grown in the spent fiber sludge hydrolysates. The cellulase activities obtained with spent hydrolysates of sulfate and sulfite fiber sludges were 2,700 and 2,900 nkat/mL, respectively. The high cellulase activities produced by using stillage and the significant ethanol concentrations produced in the second SSF suggest that onsite enzyme production and recycling of enzyme are realistic concepts that warrant further attention. © 2014 Society for Industrial Microbiology and Biotechnology.

Place, publisher, year, edition, pages
2014. Vol. 41, no 8, p. 1191-1200
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Natural Sciences
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URN: urn:nbn:se:ri:diva-187DOI: 10.1007/s10295-014-1457-9OAI: oai:DiVA.org:ri-187DiVA, id: diva2:935969
Available from: 2016-06-13 Created: 2016-06-13 Last updated: 2017-11-28Bibliographically approved

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