Sampling Considerations for Wastewater Surveillance of Antibiotic Resistance in Fecal Bacteria.Vise andre og tillknytning
2023 (engelsk)Inngår i: International Journal of Environmental Research and Public Health, ISSN 1661-7827, E-ISSN 1660-4601, Vol. 20, nr 5, artikkel-id 4555Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]
Wastewaters can be analyzed to generate population-level data for public health surveillance, such as antibiotic resistance monitoring. To provide representative data for the contributing population, bacterial isolates collected from wastewater should originate from different individuals and not be distorted by a selection pressure in the wastewater. Here we use Escherichia coli diversity as a proxy for representativeness when comparing grab and composite sampling at a major municipal wastewater treatment plant influent and an untreated hospital effluent in Gothenburg, Sweden. All municipal samples showed high E. coli diversity irrespective of the sampling method. In contrast, a marked increase in diversity was seen for composite compared to grab samples from the hospital effluent. Virtual resampling also showed the value of collecting fewer isolates on multiple occasions rather than many isolates from a single sample. Time-kill tests where individual E. coli strains were exposed to sterile-filtered hospital wastewater showed rapid killing of antibiotic-susceptible strains and significant selection of multi-resistant strains when incubated at 20 °C, an effect which could be avoided at 4 °C. In conclusion, depending on the wastewater collection site, both sampling method and collection/storage temperature could significantly impact the representativeness of the wastewater sample.
sted, utgiver, år, opplag, sider
2023. Vol. 20, nr 5, artikkel-id 4555
Emneord [en]
Escherichia coli, PhenePlateTM, composite sample, grab sample, hospital effluent, sewage, temperature, time-kill test, wastewater treatment plant influent, wastewater-based epidemiology
HSV kategori
Identifikatorer
URN: urn:nbn:se:ri:diva-67505DOI: 10.3390/ijerph20054555PubMedID: 36901565OAI: oai:DiVA.org:ri-67505DiVA, id: diva2:1804051
Merknad
This work was financially supported by the Swedish Research Council Formas (grant numbers 2018-00833 and 2021-00922) to CFF, the Centre for Antibiotic Resistance Research at the University of Gothenburg to CFF, and the Swedish state under the agreement between the Swedish Government and the county councils, the ALF agreement (grant numbers ALFGBG-717901 and ALFGBG-978722) to DGJL and CFF, respectively.
2023-10-112023-10-112023-10-12bibliografisk kontrollert