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Gene-Expression Analysis of Human Fibroblasts Affected by 3D-Printed Carboxylated Nanocellulose Constructs
RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.ORCID iD: 0000-0002-8849-1793
RISE Research Institutes of Sweden, Bioeconomy and Health, Material and Surface Design.
RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology. Gothenburg University, Sweden.ORCID iD: 0000-0002-4270-8475
RISE Research Institutes of Sweden, Bioeconomy and Health, Material and Surface Design.ORCID iD: 0000-0002-6183-2017
2023 (English)In: Bioengineering, E-ISSN 2306-5354, Vol. 10, no 1, article id 121Article in journal (Refereed) Published
Abstract [en]

Three-dimensional (3D) printing has emerged as a highly valuable tool to manufacture porous constructs. This has major advantages in, for example, tissue engineering, in which 3D scaffolds provide a microenvironment with adequate porosity for cell growth and migration as a simulation of tissue regeneration. In this study, we assessed the suitability of three cellulose nanofibrils (CNF) that were obtained through 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO)-mediated oxidation. The CNFs were obtained by applying three levels of carboxylation, i.e., 2.5, 3.8, and 6.0 mmol sodium hypochlorite (NaClO) per gram of cellulose. The CNFs exhibited different nanofibrillation levels, affecting the corresponding viscosity and 3D printability of the CNF gels (0.6 wt%). The scaffolds were manufactured by micro-extrusion and the nanomechanical properties were assessed with nanoindentation. Importantly, fibroblasts were grown on the scaffolds and the expression levels of the marker genes, which are relevant for wound healing and proliferation, were assessed in order to reveal the effect of the 3D-scaffold microenvironment of the cells. © 2023 by the authors.

Place, publisher, year, edition, pages
MDPI , 2023. Vol. 10, no 1, article id 121
Keywords [en]
3D-printing, characterization, gene expression, nanocellulose, wound dressings
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:ri:diva-63992DOI: 10.3390/bioengineering10010121Scopus ID: 2-s2.0-85146750909OAI: oai:DiVA.org:ri-63992DiVA, id: diva2:1737165
Note

Funding details: 283895, 309178; Funding details: European Commission, EC; Funding details: Norges Forskningsråd, MNET17/NMCS-1204; Funding text 1: The authors acknowledge the European Commission and the Research Council of Norway for funding part of this work through the MANUNET III program (project no. MNET17/NMCS-1204), the MedIn project (grant no. 283895), “New functionalized medical devices for surgical interventions in the pelvic cavity” and the OxyPol project (“Oxygenated biopolymers for biomedical applications”, grant no. 309178).

Available from: 2023-02-15 Created: 2023-02-15 Last updated: 2023-05-22Bibliographically approved

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Rosendahl, JenniferHåkansson, JoakimChinga-Carrasco, Gary

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