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Efficient Labeling of Nanocellulose for High-Resolution Fluorescence Microscopy Applications
McMaster University, USA.
McMaster University, USA.
McMaster University, USA.
McMaster University, USA.
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2022 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 23, no 5, p. 1981-1994Article in journal (Refereed) Published
Abstract [en]

The visualization of naturally derived cellulose nanofibrils (CNFs) and nanocrystals (CNCs) within nanocomposite materials is key to the development of packaging materials, tissue culture scaffolds, and emulsifying agents, among many other applications. In this work, we develop a versatile and efficient two-step approach based on triazine and azide-alkyne click-chem. to fluorescently label nanocelluloses with a variety of com. available dyes. We show that this method can be used to label bacterial cellulose fibrils, plant-derived CNFs, carboxymethylated CNFs, and CNCs with Cy5 and fluorescein derivatives to high degrees of labeling using minimal amounts of dye while preserving their native morphol. and crystalline structure. The ability to tune the labeling d. with this method allowed us to prepare optimized samples that were used to visualize nanostructural features of cellulose through super-resolution microscopy. The efficiency, cost-effectiveness, and versatility of this method make it ideal for labeling nanocelluloses and imaging them through advanced microscopy techniques for a broad range of applications.

Place, publisher, year, edition, pages
American Chemical Society , 2022. Vol. 23, no 5, p. 1981-1994
Keywords [en]
cellulose nanofibril emulsifying agent packaging material
National Category
Paper, Pulp and Fiber Technology
Identifiers
URN: urn:nbn:se:ri:diva-63702DOI: 10.1021/acs.biomac.1c01698Scopus ID: 2-s2.0-85129330517OAI: oai:DiVA.org:ri-63702DiVA, id: diva2:1732649
Note

This work was supported by funding through a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (RGPIN-2019-06433) and a Canadian Foundation for Innovation J.E.L.F. instrumentation grant to J.M.-M. This research made use of instrumentation within McMaster’s Centre for Advanced Light Microscopy

Available from: 2023-01-31 Created: 2023-01-31 Last updated: 2023-01-31Bibliographically approved

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Abitbol, Tiffany

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