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3D Molecular Imaging of Stratum Corneum by Mass Spectrometry Suggests Distinct Distribution of Cholesteryl Esters Compared to Other Skin Lipids
RISE Research Institutes of Sweden, Materials and Production, Product Realisation Methodology.ORCID iD: 0000-0002-2696-7215
L’Oréal Research and Innovation, France.
L’Oréal Research and Innovation, France.
RISE Research Institutes of Sweden, Bioeconomy and Health, Material and Surface Design.ORCID iD: 0000-0001-6657-1592
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2022 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, E-ISSN 1422-0067, Vol. 23, no 22, article id 13799Article in journal (Refereed) Published
Abstract [en]

The crucial barrier properties of the stratum corneum (SC) depend critically on the design and integrity of its layered molecular structure. However, analysis methods capable of spatially resolved molecular characterization of the SC are scarce and fraught with severe limitations, e.g., regarding molecular specificity or spatial resolution. Here, we used 3D time-of-flight secondary ion mass spectrometry to characterize the spatial distribution of skin lipids in corneocyte multilayer squams obtained by tape stripping. Depth profiles of specific skin lipids display an oscillatory behavior that is consistent with successive monitoring of individual lipid and corneocyte layers of the SC structure. Whereas the most common skin lipids, i.e., ceramides, C24:0 and C26:0 fatty acids and cholesteryl sulfate, are similarly organized, a distinct 3D distribution was observed for cholesteryl oleate, suggesting a different localization of cholesteryl esters compared to the lipid matrix separating the corneocyte layers. The possibility to monitor the composition and spatial distribution of endogenous lipids as well as active drug and cosmetic substances in individual lipid and corneocyte layers has the potential to provide important contributions to the basic understanding of barrier function and penetration in the SC. © 2022 by the authors.

Place, publisher, year, edition, pages
MDPI , 2022. Vol. 23, no 22, article id 13799
Keywords [en]
3D ToF-SIMS, cholesteryl esters, layer structure, lipid distribution, stratum corneum
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:ri:diva-61377DOI: 10.3390/ijms232213799Scopus ID: 2-s2.0-85142634501OAI: oai:DiVA.org:ri-61377DiVA, id: diva2:1717526
Note

Funding details: Vetenskapsrådet, VR, 2019-03731; Funding text 1: Financial support for this work was provided by the Swedish Research Council, grant No. 2019-03731 (P.S.).

Available from: 2022-12-08 Created: 2022-12-08 Last updated: 2023-06-05Bibliographically approved

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Sjövall, PeterSkedung, Lisa

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