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Biomarker imaging of single diatom cells in a microbial mat using time-of-flight secondary ion mass spectrometry (ToF-SIMS)
University of Göttingen, Germany.
University of Göttingen, Germany.
University of Göttingen, Germany.
RISE, SP – Sveriges Tekniska Forskningsinstitut, SP Kemi Material och Ytor.ORCID iD: 0000-0002-4975-6074
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2013 (English)In: Organic Geochemistry, ISSN 0146-6380, E-ISSN 1873-5290, Vol. 57, p. 23-33Article in journal (Refereed) Published
Abstract [en]

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a surface sensitive MS technique that offers a new way of studying lipid biomarkers at the microscopic level, without the need to destroy the physical integrity of the sample by extraction. We applied ToF-SIMS to a cryosection of a microbial mat and compared the results with ToF-SIMS and gas chromatography-MS (GC-MS) analysis of extracts from the same material. A wide range of lipid biomarkers was identified with ToF-SIMS in the microbial mat cryosection. Spectra and ion images revealed that individual biomarkers, including fatty acids, mono-, di- and triacylglycerols, carotenoids and chlorophyll were localized with diatom cells identified as Planothidium lanceolatum using optical microscopy. This diatom species can thus be regarded as a major lipid source within the microbial mat system. The results underpin the idea that ToF-SIMS has the potential to become an important technique for future biomarker studies, in particular for the clear cut assignment of biomarkers to distinctive morphological structures and specific microorganisms within complex biogeochemical samples.

Place, publisher, year, edition, pages
2013. Vol. 57, p. 23-33
Keywords [en]
Biogeochemical, Clear cuts, Ion images, Lipid biomarkers, Lipid sources, Microscopic levels, Morphological structures, Physical integrity, Time of flight secondary ion mass spectrometry, ToF SIMS, Triacylglycerols, Biomarkers, Chlorophyll, Extraction, Gas chromatography, Glycerol, Microorganisms, Optical microscopy, Secondary ion mass spectrometry, biomarker, diatom, mass spectrometry, microbial mat, microscopy, morphology, Bacillariophyta, Planothidium lanceolatum
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Engineering and Technology
Identifiers
URN: urn:nbn:se:ri:diva-48630DOI: 10.1016/j.orggeochem.2013.01.005Scopus ID: 2-s2.0-84874548464OAI: oai:DiVA.org:ri-48630DiVA, id: diva2:1469726
Note

Funding details: VINNOVA; Funding details: Deutsche Forschungsgemeinschaft, DFG, -/5, Th 713/3; Funding details: Deutsche Forschungsgemeinschaft, DFG; Funding text 1: We acknowledge with gratitude the reviewers, B.J. Keely, K. Mangelsdorf and H. Volk, for constructive comments that helped improve the manuscript. We also thank E. Johansson and M. Lundqvist from SKB for technical and logistic support at the Äspö Hard Rock Laboratory. The project received financial support from the German Research Foundation (DFG; grants Th 713/3, and -/5), and the Swedish Governmental Agency for Innovation Systems (VINNOVA). The study is part of the DFG Research Unit 571 “Geobiology of Organo- and Biofilms” (publication 60).

Available from: 2020-09-22 Created: 2020-09-22 Last updated: 2023-06-07Bibliographically approved

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Siljeström, SandraSjövall, Peter

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