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Determination of mechanical and rheological properties of a cell-loaded peptide gel during ECM production
Sahlgrenska Hospital, Sweden.
Chalmers University of Technology, Sweden.
Chalmers University of Technology, Sweden.
University of Gothenburg, Sweden.
Show others and affiliations
2019 (English)In: International Journal of Pharmaceutics, ISSN 0378-5173, E-ISSN 1873-3476, Vol. 563, p. 437-444Article in journal (Refereed) Published
Abstract [en]

The development of an injectable biomaterial that supports cell survival and maintains or promotes nucleus pulposus (NP) phenotype could aid delivery of cells to degenerated NPs causing low back pain. Mesenchymal cells were loaded and grown in a synthetic peptide gel, PuraMatrix ® . Cells were observed within the gels over 0–28 days, and accumulation of glycosaminoglycans were detected by histological staining. The mechanical properties of the cell-loaded constructs, and the change of the mechanical properties were studied using stress relaxation of the gels under compression and confinement. The PuraMatrix ® gel was shown to relax fast on compression indicating that the fluid could easily flow out of the gel, and thus indicating the presence of large pores/voids. The presence of these pores/voids was further supported by high mobility of dextran molecules, determined using fluorescence recovery after photo bleaching. The stress required to deform the cell-loaded constructs to a specific strain increases at day 21, at which point the presence of glycosaminoglycans within the cell-loaded constructs was also observed. The results provide evidence of changes in mechanical properties of the PuraMatrix ® matrix upon excretion of the extracellular matrix by the

Place, publisher, year, edition, pages
Elsevier B.V. , 2019. Vol. 563, p. 437-444
Keywords [en]
Extracellular matrix, GAG, Glycosaminoglycans, hMSc, PuraMatrix, Stress relaxation
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-38672DOI: 10.1016/j.ijpharm.2019.04.028Scopus ID: 2-s2.0-85064694121OAI: oai:DiVA.org:ri-38672DiVA, id: diva2:1315019
Note

Funding details: Vetenskapsrådet; Funding details: Västra Götalandsregionen; Funding details: IngaBritt och Arne Lundbergs Forskningsstiftelse; Funding text 1: The financial contributions of the VINN Excellence center SuMo Biomaterials, VINNMER, the Swedish Research Council, ALF Västra Götaland, Inga-Britt and Arne Lundberg Foundation, the Chalmers Area of Advance Materials and the Dr. Felix Neuberghs Foundation are gratefully acknowledged.

Available from: 2019-05-10 Created: 2019-05-10 Last updated: 2019-05-10Bibliographically approved

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