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Coating 3D Printed Polycaprolactone Scaffolds with Nanocellulose Promotes Growth and Differentiation of Mesenchymal Stem Cells
University of Bergen, Norway.
University of Bergen, Norway.
University of Bergen, Norway; University of Tampere, Finland.
University of Bergen, Norway.
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2018 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 19, no 11, p. 4307-4319Article in journal (Refereed) Published
Abstract [en]

3D printed polycaprolactone (PCL) has potential as a scaffold for bone tissue engineering, but the hydrophobic surface may hinder optimal cell responses. The surface properties can be improved by coating the scaffold with cellulose nanofibrils material (CNF), a multiscale hydrophilic biocompatible biomaterial derived from wood. In this study, human bone marrow-derived mesenchymal stem cells were cultured on tissue culture plates (TCP) and 3D printed PCL scaffolds coated with CNF. Cellular responses to the surfaces (viability, attachment, proliferation, and osteogenic differentiation) were documented. CNF significantly enhanced the hydrophilic properties of PCL scaffolds and promoted protein adsorption. Live/dead staining and lactate dehydrogenase release assays confirmed that CNF did not inhibit cellular viability. The CNF between the 3D printed PCL strands and pores acted as a hydrophilic barrier, enhancing cell seeding efficiency, and proliferation. CNF supported the formation of a well-organized actin cytoskeleton and cellular production of vinculin protein on the surfaces of TCP and PCL scaffolds. Moreover, CNF-coated surfaces enhanced not only alkaline phosphatase activity, but also collagen Type-I and mineral formation. It is concluded that CNF coating enhances cell attachment, proliferation, and osteogenic differentiation and has the potential to improve the performance of 3D printed PCL scaffolds for bone tissue engineering.

Place, publisher, year, edition, pages
2018. Vol. 19, no 11, p. 4307-4319
Keywords [en]
Biocompatibility, Bone, Cell culture, Cell engineering, Cellulose, Coatings, Hydrophilicity, Hydrophobicity, Nanocellulose, Phosphatases, Polycaprolactone, Scaffolds (biology), Stem cells, Surface chemistry, Tissue, Tissue culture, Transmission control protocol, Wood, Alkaline phosphatase activity, Bone tissue engineering, Cellulose nanofibrils, Human bone marrow derived mesenchymal stem cells, Hydrophilic properties, Lactate dehydrogenase, Osteogenic differentiation, Polycaprolactone scaffolds, 3D printers
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Natural Sciences
Identifiers
URN: urn:nbn:se:ri:diva-35909DOI: 10.1021/acs.biomac.8b01194Scopus ID: 2-s2.0-85055577081OAI: oai:DiVA.org:ri-35909DiVA, id: diva2:1261112
Available from: 2018-11-06 Created: 2018-11-06 Last updated: 2019-03-06Bibliographically approved

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Heggset, Ellinor BSyverud, Kristin

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