Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
A non-linear model of hydrogen production by Caldicellulosiruptor saccharolyticus for diauxic-like consumption of lignocellulosic sugar mixtures
RISE - Research Institutes of Sweden, Samhällsbyggnad, Energi och cirkulär ekonomi.ORCID-id: 0000-0002-5758-4137
Lund University, Sweden.
Lund University, Sweden.
RISE - Research Institutes of Sweden, Samhällsbyggnad, Energi och cirkulär ekonomi.ORCID-id: 0000-0002-0359-3652
2018 (engelsk)Inngår i: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 11, artikkel-id 175Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background

Caldicellulosiruptor saccharolyticus is an attractive hydrogen producer suitable for growth on various lignocellulosic substrates. The aim of this study was to quantify uptake of pentose and hexose monosaccharides in an industrial substrate and to present a kinetic growth model of C. saccharolyticus that includes sugar uptake on defined and industrial media. The model is based on Monod and Hill kinetics extended with gas-to-liquid mass transfer and a cybernetic approach to describe diauxic-like growth.

Results

Mathematical expressions were developed to describe hydrogen production by C. saccharolyticus consuming glucose, xylose, and arabinose. The model parameters were calibrated against batch fermentation data. The experimental data included four different cases: glucose, xylose, sugar mixture, and wheat straw hydrolysate (WSH) fermentations. The fermentations were performed without yeast extract. The substrate uptake rate of C. saccharolyticus on single sugar-defined media was higher on glucose compared to xylose. In contrast, in the defined sugar mixture and WSH, the pentoses were consumed faster than glucose. Subsequently, the cultures entered a lag phase when all pentoses were consumed after which glucose uptake rate increased. This phenomenon suggested a diauxic-like behavior as was deduced from the successive appearance of two peaks in the hydrogen and carbon dioxide productivity. The observation could be described with a modified diauxic model including a second enzyme system with a higher affinity for glucose being expressed when pentose saccharides are consumed. This behavior was more pronounced when WSH was used as substrate.

Conclusions

The previously observed co-consumption of glucose and pentoses with a preference for the latter was herein confirmed. However, once all pentoses were consumed, C. saccharolyticus most probably expressed another uptake system to account for the observed increased glucose uptake rate. This phenomenon could be quantitatively captured in a kinetic model of the entire diauxic-like growth process. Moreover, the observation indicates a regulation system that has fundamental research relevance, since pentose and glucose uptake in C. saccharolyticus has only been described with ABC transporters, whereas previously reported diauxic growth phenomena have been correlated mainly to PTS systems for sugar uptake.

sted, utgiver, år, opplag, sider
2018. Vol. 11, artikkel-id 175
Emneord [en]
Caldicellulosiruptor saccharolyticus – Hydrogen – Kinetic growth model – Glucose uptake – Xylose uptake – Diauxic
HSV kategori
Identifikatorer
URN: urn:nbn:se:ri:diva-34067DOI: 10.1186/s13068-018-1171-3Scopus ID: 2-s2.0-85049011727OAI: oai:DiVA.org:ri-34067DiVA, id: diva2:1231262
Forskningsfinansiär
Swedish Energy Agency, 31090-2Tilgjengelig fra: 2018-07-06 Laget: 2018-07-06 Sist oppdatert: 2019-01-07bibliografisk kontrollert

Open Access i DiVA

fulltext(1365 kB)196 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1365 kBChecksum SHA-512
77c22e8522cbdd4a3bb8616df73231d0eee4d5fb83809034adcea455b69e5d2bc773c2c102801be6f10be767c126d15ad1c86e2b853022165dd1702fc1a937a7
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstScopus

Person

Björkmalm, JohannaWillquist, Karin

Søk i DiVA

Av forfatter/redaktør
Björkmalm, JohannaWillquist, Karin
Av organisasjonen
I samme tidsskrift
Biotechnology for Biofuels

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 196 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 168 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
v. 2.43.0