The lighting in the workplace is known to have a significant effect on the workers’ well-being and alertness. It also affects sleep/wake-cycles and mood. The aim of this pilot study is to investigate a method to evaluate non-visual effects of variable lighting in workplaces using both self-assessed and physical data. The lighting in two offices was varied according to pre-programmed schedules, including daylight simulating scenario for two weeks and activity promoting scenario for two weeks. The method was successful and provided interesting results on the measured physical data. The resting pulse was lowered and the sleep quality improved for the test subjects during the weeks of dynamic office lighting.