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Viral detection and quantification in a digital droplet microfluidic lab-in-a-fiber device
KTH Royal Institute of Technology, Sweden.
KTH Royal Institute of Technology, Sweden.
KTH Royal Institute of Technology, Sweden.
KTH Royal Institute of Technology, Sweden.
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2021 (Engelska)Ingår i: Proceedings of SPIE - The International Society for Optical Engineering, SPIE , 2021Konferensbidrag, Publicerat paper (Refereegranskat)
Abstract [en]

In this work, we present the design and fabrication of a fiber device that performs digital droplet microfluidics for molecular diagnostics. A variety of fibers and capillaries were used to build three connected modules dedicated to droplet generation, incubation, and fluorescence detection which enables a uniaxial arrangement. This is in contrast to the traditional 2-dimensional lab-on-a-chip architecture. We characterize our fiber device using a fluorescein dilution series. Our observed detection limit is on the order of 10 nM fluorescein. We demonstrate our all-fiber device for the fluorescence readout after loop-mediated isothermal amplification (LAMP) of synthetic SARS-CoV-2. Our results suggest that this fiber device can successfully distinguish between positive and negative samples in molecular diagnostics. We propose that our fiber device offers benefits over microfluidic chip techniques such as easier optical integration, much simpler sample loading, and faster diagnosis with high specificity and sensitivity. Keywords: All-fiber device, microfluidics, optofluidics, loop-mediated isothermal amplification (LAMP), molecular diagnostics, SARS-CoV2.

Ort, förlag, år, upplaga, sidor
SPIE , 2021.
Nyckelord [en]
Digital microfluidics, Diseases, Drops, Fluorescence, Isotherms, Microstructure, Chip architecture, Droplet generation, Droplet microfluidics, Fluorescence detection, Loop mediated isothermal amplifications, Microfluidic chip, Molecular diagnostics, Optical integration, Optical fibers
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:ri:diva-55491DOI: 10.1117/12.2590850Scopus ID: 2-s2.0-85109212481ISBN: 9781510643802 (tryckt)OAI: oai:DiVA.org:ri-55491DiVA, id: diva2:1582865
Konferens
Micro-structured and Specialty Optical Fibres VII 2021, 19 April 2021 through 23 April 2021
Tillgänglig från: 2021-08-04 Skapad: 2021-08-04 Senast uppdaterad: 2021-08-04Bibliografiskt granskad

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Margulis, Walter

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