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Electrochemical bacterial detection using poly(3-aminophenylboronic acid)-based imprinted polymer
Linköping University, Sweden.
Ordu University, Turkey.
Linköping University, Sweden.
RISE - Research Institutes of Sweden, ICT, Acreo. Linköping University, Sweden.
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2017 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 93, s. 87-93Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Biosensors can deliver the rapid bacterial detection that is needed in many fields including food safety, clinical diagnostics, biosafety and biosecurity. Whole-cell imprinted polymers have the potential to be applied as recognition elements in biosensors for selective bacterial detection. In this paper, we report on the use of 3-aminophenylboronic acid (3-APBA) for the electrochemical fabrication of a cell-imprinted polymer (CIP). The use of a monomer bearing a boronic acid group, with its ability to specifically interact with cis-diol, allowed the formation of a polymeric network presenting both morphological and chemical recognition abilities. A particularly beneficial feature of the proposed approach is the reversibility of the cis-diol-boronic group complex, which facilitates easy release of the captured bacterial cells and subsequent regeneration of the CIP. Staphylococcus epidermidis was used as the model target bacteria for the CIP and electrochemical impedance spectroscopy (EIS) was explored for the label-free detection of the target bacteria. The modified electrodes showed a linear response over the range of 103–107 cfu/mL. A selectivity study also showed that the CIP could discriminate its target from non-target bacteria having similar shape. The CIPs had high affinity and specificity for bacterial detection and provided a switchable interface for easy removal of bacterial cell.

Ort, förlag, år, upplaga, sidor
2017. Vol. 93, s. 87-93
Nyckelord [en]
3-Aminophenylboronic acid, Electrochemical impedance spectroscopy, Label-free detection, Staphylococcus epidermidis, Whole-cell imprinted polymers, Bacteria, Biosensors, Complex networks, Polymers, Spectroscopy, Chemical recognition, Clinical diagnostics, Electrochemical fabrication, Imprinted polymers, Rapid bacterial detections, Chemical detection
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Naturvetenskap
Identifikatorer
URN: urn:nbn:se:ri:diva-29307DOI: 10.1016/j.bios.2016.09.088Scopus ID: 2-s2.0-85001760499OAI: oai:DiVA.org:ri-29307DiVA, id: diva2:1095210
Tillgänglig från: 2017-05-12 Skapad: 2017-05-12 Senast uppdaterad: 2018-02-26Bibliografiskt granskad

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