Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Cytotoxicity tests of cellulose nanofibril-based structures
NTNU Norwegian University of Science and Technology, Norway.
RISE., Innventia, PFI – Paper and Fiber Research Institute. NTNU Norwegian University of Science and Technology, Norway.ORCID-id: 0000-0003-2271-3637
ISTEC-CNR, Italy.
RISE., Innventia, PFI – Paper and Fiber Research Institute.ORCID-id: 0000-0002-6183-2017
2013 (engelsk)Inngår i: Cellulose, ISSN 0969-0239, E-ISSN 1572-882X, Vol. 20, nr 4, s. 1765-1775Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Cellulose nanofibrils based on wood pulp fibres are most promising for biomedical applications. Bacterial cellulose has been suggested for some medical applications and is presently used as wound dressing. However, cost-efficient processes for mass production of bacterial cellulose are lacking. Hence, fibrillation of cellulose wood fibres is most interesting, as the cellulose nanofibrils can efficiently be produced in large quantities. However, the utilization of cellulose nanofibrils from wood requires a thorough verification of its biocompatibility, especially with fibroblast cells which are important in regenerative tissue and particularly in wound healing. The cellulose nanofibril structures used in this study were based on Eucalyptus and Pinus radiata pulp fibres. The nanofibrillated materials were manufactured using a homogenizer without pre-treatment and with 2,2,6,6-tetramethylpiperidine-1-oxy radical as pre-treatment, thus yielding nanofibrils low and high level of anionic charge, respectively. From these materials, two types of nanofibril-based structures were formed; (1) thin and dense structures and (2) open and porous structures. Cytotoxicity tests were applied on the samples, which demonstrated that the nanofibrils do not exert acute toxic phenomena on the tested fibroblast cells (3T3 cells). The cell membrane, cell mitochondrial activity and the DNA proliferation remained unchanged during the tests, which involved direct and indirect contact between the nano-structured materials and the 3T3 cells. Some samples were modified using the crosslinking agent polyethyleneimine (PEI) or the surfactant cetyl trimethylammonium bromide (CTAB). The sample modified with CTAB showed a clear toxic behaviour, having negative effects on cell survival, viability and proliferation. CTAB is an antimicrobial component, and thus this result was as expected. The sample crosslinked with PEI also had a significant reduction in cell viability indicating a reduction in DNA proliferation. We conclude that the neat cellulose nanostructured materials tested in this study are not toxic against fibroblasts cells. This is most important as nano-structured materials based on nanofibrils from wood pulp fibres are promising as substrate for regenerative medicine and wound healing.

sted, utgiver, år, opplag, sider
2013. Vol. 20, nr 4, s. 1765-1775
HSV kategori
Identifikatorer
URN: urn:nbn:se:ri:diva-9619DOI: 10.1007/s10570-013-9948-9Scopus ID: 2-s2.0-84881022355OAI: oai:DiVA.org:ri-9619DiVA, id: diva2:968372
Tilgjengelig fra: 2016-09-12 Laget: 2016-09-12 Sist oppdatert: 2023-05-25bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstScopus

Person

Syverud, KristinChinga-Carrasco, Gary

Søk i DiVA

Av forfatter/redaktør
Syverud, KristinChinga-Carrasco, Gary
Av organisasjonen
I samme tidsskrift
Cellulose

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 526 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
v. 2.43.0