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Factors affecting detoxification of softwood enzymatic hydrolysates using sodium dithionite
Umeå University, Sweden.
Umeå University, Sweden.
RISE Research Institutes of Sweden, Bioekonomi och hälsa, Bioraffinaderi och energi.ORCID-id: 0009-0009-0187-2779
Umeå University, Sweden.
2021 (engelsk)Inngår i: Processes, ISSN 2227-9717, Vol. 9, nr 5, artikkel-id 887Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Conditioning of lignocellulosic hydrolysates with sulfur oxyanions, such as dithionite, is one of the most potent methods to improve the fermentability by counteracting effects of inhibitory by-products generated during hydrothermal pretreatment under acidic conditions. The effects of pH, treatment temperature, and dithionite dosage were explored in experiments with softwood hydrolysates, sodium dithionite, and Saccharomyces cerevisiae yeast. Treatments with dithionite at pH 5.5 or 8.5 gave similar results with regard to ethanol productivity and yield on initial glucose, and both were always at least ~20% higher than for treatment at pH 2.5. Experiments in the dithionite concentration range 5.0–12.5 mM and the temperature range 23–110◦ C indicated that treatment at around 75◦ C and using intermediate dithionite dosage was the best option (p ≤ 0.05). The investigation indicates that selection of the optimal temperature and dithionite dosage offers great benefits for the efficient fermentation of hydrolysates from lignin-rich biomass, such as softwood residues. © 2021 by the authors. 

sted, utgiver, år, opplag, sider
MDPI AG , 2021. Vol. 9, nr 5, artikkel-id 887
Emneord [en]
Cellulosic ethanol, Conditioning, Detoxification, Inhibitors, Lignocellulose biorefining, Sodium dithionite
HSV kategori
Identifikatorer
URN: urn:nbn:se:ri:diva-53527DOI: 10.3390/pr9050887Scopus ID: 2-s2.0-85106925621OAI: oai:DiVA.org:ri-53527DiVA, id: diva2:1568283
Merknad

Funding details: Energimyndigheten, P41285-1, P47516-1; Funding details: Kempestiftelserna; Funding text 1: Funding: This research was funded by Swedish Energy Agency (P41285-1, P47516-1), Kempe Foundations, and the Bio4Energy research environment (www.bio4energy.se, accessed on 23 April 2021).

Tilgjengelig fra: 2021-06-17 Laget: 2021-06-17 Sist oppdatert: 2023-12-05bibliografisk kontrollert

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