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DNA biosensors implemented on PNA-functionalized microstructured optical fibers Bragg gratings
University of Parma, Italy.
University of Parma, Italy.
University of Parma, Italy.
University of Parma, Italy.
Vise andre og tillknytning
2013 (engelsk)Inngår i: Proceedings of SPIE - The International Society for Optical Engineering, 2013, artikkel-id 877502Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

A novel DNA sensing platform based on a Peptide Nucleic Acid-functionalized Microstructured Optical Fibers gratings has been demonstrated. The inner surface of different MOFs has been functionalized using PNA probes, OligoNucleotides mimic that are well suited for specific DNA target sequences detection. The hybrid sensing systems were tested for optical DNA detection of targets of relevance in biomedical application, using the cystic fibrosis gene mutation, and food-analysis, using the genomic DNA from genetic modified organism soy flour. After the solutions of DNA molecules has been infiltrated inside the fibers capillaries and hybridization has occurred, oligonucleotidefunctionalized gold nanoparticles were infiltrated and used to form a sandwich-like system to achieve signal amplification. Spectral measurements of the reflected signal reveal a clear wavelength shift of the reflected modes when the infiltrated complementary DNA matches with the PNA probes placed on the inner fiber surface. Measurements have also been made using the mismatched DNA solution for the c, containing a single nucleotide polymorphism, showing no significant changes in the reflected spectrum. Several experiments have been carried out demonstrating the reproducibility of the results and the high selectivity of the sensors, showing the simplicity and the potential of this approach.

sted, utgiver, år, opplag, sider
2013. artikkel-id 877502
Emneord [en]
Biosensors, Cystic Fibrosis (CF), DNA detection, Fiber Bragg Gratings (FBGs), Genomic Genetically Modified Organism DNA, Gold Nanoparticles (AuNPs), Microstructured Optical Fibers (MOFs), Peptide Nucleic Acid (PNA), Cystic fibrosis, Genetically modified organisms, Gold nanoparticle, Microstructured optical fibers, Peptide nucleic acid, DNA, Fiber Bragg gratings, Genes, Gold, Medical applications, Metal nanoparticles, Oligonucleotides, Peptides, Probes, Sensors, DNA sequences
HSV kategori
Identifikatorer
URN: urn:nbn:se:ri:diva-49990DOI: 10.1117/12.2016684Scopus ID: 2-s2.0-84880869666ISBN: 9780819495778 (tryckt)OAI: oai:DiVA.org:ri-49990DiVA, id: diva2:1479336
Konferanse
Micro-Structured and Specialty Optical Fibres II, 15 April 2013 through 17 April 2013, Prague
Tilgjengelig fra: 2020-10-26 Laget: 2020-10-26 Sist oppdatert: 2020-12-01bibliografisk kontrollert

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