Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Structure and Composition of Native Membrane Derived Polymer-Supported Lipid Bilayers
Chalmers University of Technology, Sweden.
RISE - Research Institutes of Sweden, Biovetenskap och material, Yta, process och formulering. Chalmers University of Technology, Sweden.
Chalmers University of Technology, Sweden.
Institute Laue-Langevin, France ; Imperial College London, UK.
Vise andre og tillknytning
2018 (engelsk)Inngår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, nr 21, s. 13065-13072Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Over the last two decades, supported lipid bilayers (SLBs) have been extensively used as model systems to study cell membrane structure and function. While SLBs have been traditionally produced from simple lipid mixtures, there has been a recent surge in compositional complexity to better mimic cellular membranes and thereby bridge the gap between classic biophysical approaches and cell experiments. To this end, native cellular membrane derived SLBs (nSLBs) have emerged as a new category of SLBs. As a new type of biomimetic material, an analytical workflow must be designed to characterize its molecular composition and structure. Herein, we demonstrate how a combination of fluorescence microscopy, neutron reflectometry, and secondary ion mass spectrometry offers new insights on structure, composition, and quality of nSLB systems formed using so-called hybrid vesicles, which are a mixture of native membrane material and synthetic lipids. With this approach, we demonstrate that the nSLB formed a continuous structure with complete mixing of the synthetic and native membrane components and a molecular stoichiometry that essentially mirrors that of the hybrid vesicles. Furthermore, structural investigation of the nSLB revealed that PEGylated lipids do not significantly thicken the hydration layer between the bilayer and substrate when on silicon substrates; however, nSLBs do have more topology than their simpler, purely synthetic counterparts. Beyond new insights regarding the structure and composition of nSLB systems, this work also serves to guide future researchers in producing and characterizing nSLBs from their cellular membrane of choice.

sted, utgiver, år, opplag, sider
2018. Vol. 90, nr 21, s. 13065-13072
Emneord [en]
Biomimetics, Cell membranes, Cytology, Fluorescence microscopy, Lipid bilayers, Lipids, Mixtures, Secondary ion mass spectrometry, Substrates, Cellular membranes, Compositional complexity, Continuous structures, Molecular compositions, Neutron reflectometry, Silicon substrates, Structural investigation, Supported lipid bilayers, Structure (composition)
HSV kategori
Identifikatorer
URN: urn:nbn:se:ri:diva-36374DOI: 10.1021/acs.analchem.8b04110Scopus ID: 2-s2.0-85056285757OAI: oai:DiVA.org:ri-36374DiVA, id: diva2:1266232
Tilgjengelig fra: 2018-11-27 Laget: 2018-11-27 Sist oppdatert: 2019-06-20bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstScopus

Personposter BETA

Sjövall, Peter

Søk i DiVA

Av forfatter/redaktør
Sjövall, Peter
Av organisasjonen
I samme tidsskrift
Analytical Chemistry

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 6 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
v. 2.35.7